RUNX3 Polyclonal Antibody | anti-RUNX3 antibody

Anti-RUNX3 Antibody

Cat. Num. AAA178227

• Gene Names: RUNX3; AML2; CBFA3; PEBP2aC
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: RUNX3; Polyclonal Antibody; Anti-RUNX3 Antibody; Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Acute myeloid leukemia gene 2; AML 2; AML2; CBF alpha 3; CBF-alpha-3; CBFA 3; CBFA3; Core binding factor alpha 3 subunit; core binding factor; Core binding factor runt domain alpha subunit 3; Core binding factor subunit alpha 3; core-binding factor; Core-binding factor subunit alpha-3; FLJ34510; MGC16070; Oncogene AML 2; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Pebp2a3; PEBP2aC; Polyomavirus enhancer binding protein 2 alpha C subunit; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain alpha subunit 3; runt related transcription factor 3; RUNX 3; Runx3; RUNX3_HUMAN; SL3 3 enhancer factor 1 alpha C subunit; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core binding factor alpha C subunit; SL3/AKV core-binding factor alpha C subunit; Transcription factor AML2; runt-related transcription factor 3; anti-RUNX3 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Sequence Length: 429

• Applicable Applications for anti-RUNX3 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot Concentration: 0.1-0.5ug/ml; Immunohistochemistry (IHC) Paraffin Concentration: 0.5-1ug/ml
• Immunogen: E Coli-derived human RUNX3 recombinant protein (Position: M128-Y270). Human RUNX3 shares 93% amino acid (aa) sequence identity with mouse RUNX3.
• Ig Type: Rabbit IgG
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti- RUNX3 Picoband antibody, MBS178227, Western blottingAll lanes: Anti RUNX3 (MBS178227) at 0.5ug/mlLane 1: A431 Whole Cell Lysate at 40ugLane 2: U20S Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD)

Immunohistochemistry (IHC)

(Anti- RUNX3 Picoband antibody, MBS178227, IHC(P)IHC(P): Mouse Spleen Tissue )

Immunohistochemistry (IHC)

(Anti- RUNX3 Picoband antibody, MBS178227, IHC(P)IHC(P): Rat Spleen Tissue )

Immunohistochemistry (IHC)

(Anti- RUNX3 Picoband antibody, MBS178227, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti- RUNX3 Picoband antibody, MBS178227, IHC(P)IHC(P): Human Lung Cancer Tissue )

Related Product Information for anti-RUNX3 antibody

Description: Rabbit IgG polyclonal antibody for Runt-related transcription factor 3(RUNX3) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Background: Runt-related transcription factor 3, also called AML2 is a protein that in humans is encoded by the RUNX3 gene. The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family. By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11. RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes. It may be involved in the control of cellular proliferation and/or differentiation.

References

1. Brenner, O., Levanon, D., Negreanu, V., Golubkov, O., Fainaru, O., Woolf, E., Groner, Y. Loss of Runx3 function in leukocytes is associated with spontaneously developed colitis and gastric mucosal hyperplasia. Proc. Nat. Acad. Sci. 101: 16016-16021, 2004. 2. Fainaru, O., Shseyov, D., Hantisteanu, S., Groner, Y. Accelerated chemokine receptor 7-mediated dendritic cell migration in Runx3 knockout mice and the spontaneous development of asthma-like disease. Proc. Nat. Acad. Sci. 102: 10598-10603, 2005. 3. Ito, K., Liu, Q., Salto-Tellez, M., Yano, T., Tada, K., Ida, H., Huang, C., Shah, N., Inoue, M., Rajnakova, A., Hiong, K. C., Peh, B. K., Han, H. C., Ito, T., Teh, M., Yeoh, K. G., Ito, Y. RUNX3, a novel tumor suppressor, is frequently inactivated in gastric cancer by protein mislocalization. Cancer Res. 65: 7743-7750, 2005. 4. Li, Q.-L., Ito, K., Sakakura, C., Fukamachi, H., Inoue, K., Chi, X.-Z., Lee, K.-Y., Nomura, S., Lee, C.-W., Han, S.-B., Kim, H.-M., Kim, W.-J., and 15 others. Causal relationship between the loss of RUNX3 expression and gastric cancer. Cell 109: 113-124, 2002.

NCBI and Uniprot Product Information

• NCBI GI #: 72534652
• NCBI GeneID: 864
• NCBI Accession #: NP_001026850.1
• NCBI GenBank Nucleotide #: NM_001031680.2
• UniProt Accession #: Q13761; Q12969; Q13760; B1AJV5
• Molecular Weight: 45,922 Da
• NCBI Official Full Name: runt-related transcription factor 3 isoform 1
• NCBI Official Synonym Full Names: runt related transcription factor 3
• NCBI Official Symbol: RUNX3
• NCBI Official Synonym Symbols: AML2; CBFA3; PEBP2aC
• NCBI Protein Information: runt-related transcription factor 3
• UniProt Protein Name: Runt-related transcription factor 3
• Protein Family: Runt-related transcription factor
• UniProt Gene Name: RUNX3
• UniProt Synonym Gene Names: AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C
• UniProt Entry Name: RUNX3_HUMAN

NCBI Description

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

AML2: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA- binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: cytoplasm; nuclear chromatin; nucleus

Molecular Function: ATP binding; protein binding; transcription factor activity

Biological Process: chondrocyte differentiation; hemopoiesis; negative regulation of cell cycle; negative regulation of epithelial cell proliferation; negative regulation of transcription from RNA polymerase II promoter; ossification; peripheral nervous system neuron development; positive regulation of transcription, DNA-dependent; protein amino acid phosphorylation; regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter