Rabbit anti-Human RAD52 Polyclonal Antibody | anti-RAD52 antibody

Anti-Human RAD52 Antibody

Cat. Num. AAA4157753

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: RAD52; Polyclonal Antibody; Anti-Human RAD52 Antibody; Human RAD52; anti-RAD52 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human RAD52 protein. Cross reactivity with RAD52 protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.
• Purity/Purification: Affinity Purified
• Form/Format: Liquid; PBS, pH 7.4 with 0.05% sodium azide.
• Concentration: 1ug/ul (varies by lot)
• Sequence Length: 76

• Applicable Applications for anti-RAD52 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: This affinity purified antibody has been tested for use in western blotting using HeLa nuclear extracts. The antibody is also functional by ELISA. Dilutions for western blotting represent a starting point dilution and further optimization may be required. The antibody detects a band of approximately 63.0 kDa (predicted molecular weight: 46.3 kDa). Specific band detection by western blot is blocked by peptide competition by pre-incubating the antibody with the immunizing peptide prior to reaction with the membrane. Reactivity in other immunoassays is unknown.
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.; For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-RAD52 antibody

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. Four alternatively spliced transcript variants encoding different isoforms have been reported for this gene. The alpha variant encodes the longest isoform that contains an identical N-terminus, but a distinct C-terminus, as compared to other isoforms (beta, delta, and gamma).

NCBI and Uniprot Product Information

• NCBI GI #: 6176321
• NCBI GeneID: 5893
• NCBI Official Full Name: RAD52, partial
• NCBI Official Synonym Full Names: RAD52 homolog, DNA repair protein
• NCBI Official Symbol: RAD52
• NCBI Protein Information: DNA repair protein RAD52 homolog
• Protein Family: RAD52 motif-containing protein

NCBI Description

The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]