Human Serine protease hepsin ELISA Kit | HPN elisa kit

Human Serine protease hepsin ELISA Kit

Cat. Num. AAA763620

• Gene Names: HPN; TMPRSS1
• Reactivity: Human
• Synonyms: Serine protease hepsin; Human Serine protease hepsin ELISA Kit; HPN/TMPRSS1/hepsin/serine protease hepsin/serine protease hepsin catalytic chain/serine protease hepsin non-catalytic chain/Transmembrane protease serine 1/transmembrane protease; serine 1; HPN elisa kit

• Reactivity: Human
• Sequence Length: 417

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.78-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for HPN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human HPN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HPN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HPN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HPN can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 33695155
• NCBI GeneID: 3249
• NCBI Accession #: NP_892028.1
• NCBI GenBank Nucleotide #: NM_182983.2
• Molecular Weight: 45,011 Da
• NCBI Official Full Name: serine protease hepsin
• NCBI Official Synonym Full Names: hepsin
• NCBI Official Symbol: HPN
• NCBI Official Synonym Symbols: TMPRSS1
• NCBI Protein Information: serine protease hepsin
• UniProt Protein Name: Serine protease hepsin
• Protein Family: Serine protease hepsin
• UniProt Gene Name: HPN
• UniProt Synonym Gene Names: TMPRSS1
• UniProt Entry Name: HEPS_HUMAN

NCBI Description

This gene encodes a type II transmembrane serine protease that may be involved in diverse cellular functions, including blood coagulation and the maintenance of cell morphology. Expression of the encoded protein is associated with the growth and progression of cancers, particularly prostate cancer. The protein is cleaved into a catalytic serine protease chain and a non-catalytic scavenger receptor cysteine-rich chain, which associate via a single disulfide bond. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013]

Uniprot Description

HPN: Plays an essential role in cell growth and maintenance of cell morphology. Belongs to the peptidase S1 family.

Protein type: Membrane protein, integral; Protease; EC 3.4.21.106

Chromosomal Location of Human Ortholog: 19q13.12

Cellular Component: cell soma; cell surface; endoplasmic reticulum membrane; integral to membrane; integral to plasma membrane; intercellular junction; nuclear membrane; plasma membrane

Molecular Function: calcium-activated potassium channel activity; peptidase activity; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: cholesterol homeostasis; detection of mechanical stimulus involved in sensory perception of sound; negative regulation of apoptosis; negative regulation of epithelial cell proliferation; positive regulation of cell growth; proteolysis; regulation of cell shape