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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Mediator of RNA polymerase II transcription subunit 18 (MED18) Standard Curve.)

Human Mediator Of RNA Polymerase II Transcription Subunit 18 (MED18) ELISA Kit | MED18 elisa kit

Human Mediator Of RNA Polymerase II Transcription Subunit 18 (MED18) ELISA Kit

Gene Names
MED18; SRB5; p28b
Reactivity
Human
Synonyms
Mediator Of RNA Polymerase II Transcription Subunit 18 (MED18); Human Mediator Of RNA Polymerase II Transcription Subunit 18 (MED18) ELISA Kit; MED18; FLJ20045; p28b; TRAP/mediator complex subunit p28b; mediator of RNA polymerase transcription; subunit 18 homolog; MED18 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED18. No significant cross-reactivity or interference between Human MED18 and analogues was observed.
Sequence Length
208
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Mediator of RNA polymerase II transcription subunit 18 (MED18) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Human Mediator of RNA polymerase II transcription subunit 18 (MED18) Standard Curve.)
Related Product Information for MED18 elisa kit
Description: This Human Mediator of RNA polymerase II transcription subunit 18 (MED18) ELISA Kit employs a two-site sandwich ELISA to quantitate MED18 in samples. An antibody specific for MED18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MED18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED18 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mediator of RNA polymerase II transcription subunit 18
NCBI Official Synonym Full Names
mediator complex subunit 18
NCBI Official Symbol
MED18
NCBI Official Synonym Symbols
SRB5; p28b
NCBI Protein Information
mediator of RNA polymerase II transcription subunit 18
UniProt Protein Name
Mediator of RNA polymerase II transcription subunit 18
UniProt Gene Name
MED18
UniProt Entry Name
MED18_HUMAN

NCBI Description

MED18 is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (Sato et al., 2003 [PubMed 12584197]).[supplied by OMIM, Oct 2008]

Uniprot Description

MED18: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 18 family.

Protein type: Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: Srb-mediator complex

Molecular Function: protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Research Articles on MED18

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Product Notes

The Human MED18 med18 (Catalog #AAA9715328) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715328 ELISA Kit recognizes Human MED18. It is sometimes possible for the material contained within the vial of "Mediator Of RNA Polymerase II Transcription Subunit 18 (MED18), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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