Rabbit RAB14 Polyclonal Antibody | anti-RAB14 antibody

RAB14 Polyclonal Antibody

Cat. Num. AAA3006769

• Gene Names: RAB14; FBP; RAB-14
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAB14; Polyclonal Antibody; RAB14 Polyclonal Antibody; Ras-related protein Rab-14; anti-RAB14 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAB14 polyclonal antibody detects endogenous levels of RAB14 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 215

• Applicable Applications for anti-RAB14 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RAB14.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAB14 polyclonal antibody at 1:500 dilutionLane1:Jurkat whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-RAB14 antibody

The Ras-related superfamily of guanine nucleotide binding proteins includes the R-Ras, Rap, Ral/Rec and Rho/Rab subfamilies all of which are thought to play an important role in either endocytosis or in biosynthetic protein transport. The process of transporting newly synthesized proteins from the endoplasmic reticulum (ER) to various stacks of the Golgi complex and to secretory vesicles involves the movement of carrier vesicles and requires Rab protein function. Rab proteins are also an integral part of endocytic pathways. Rab 14, also known as FBP, is a 215 amino acid protein that is lipid-anchored to the cytoplasmic side of the cell membrane. One of several members of the Rab subfamily of small GTPases, Rab 14 is thought to be involved in vesicular trafficking and neurotransmitter release throughout the body and is expressed at high levels in brain, lung, kidney, spleen and thymus.

NCBI and Uniprot Product Information

• NCBI GI #: 19923483
• NCBI GeneID: 51552
• NCBI Accession #: NP_057406.2
• NCBI GenBank Nucleotide #: NP_057406.2
• UniProt Accession #: P61106
• Molecular Weight: ~ 22kDa
• NCBI Official Full Name: ras-related protein Rab-14
• NCBI Official Synonym Full Names: RAB14, member RAS oncogene family
• NCBI Official Symbol: RAB14
• NCBI Official Synonym Symbols: FBP; RAB-14
• NCBI Protein Information: ras-related protein Rab-14
• UniProt Protein Name: Ras-related protein Rab-14
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB14
• UniProt Entry Name: RAB14_HUMAN

NCBI Description

RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM, Mar 2009]

Uniprot Description

RAB14: a protein of the GTPase superfamily, Rab family. May be involved in vesicular trafficking and neurotransmitter release. Localized to the inner face of the cell membrane by a lipid-anchor.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: Golgi apparatus; Golgi stack; cytoplasmic vesicle membrane; nuclear envelope-endoplasmic reticulum network; intracellular membrane-bound organelle; rough endoplasmic reticulum; lysosomal membrane; lysosome; early endosome; phagocytic vesicle; cytosol; Golgi membrane; trans-Golgi network transport vesicle; recycling endosome; phagocytic vesicle membrane; perinuclear region of cytoplasm; early endosome membrane; late endosome; plasma membrane; intracellular

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; myosin V binding

Biological Process: vesicle-mediated transport; intracellular protein transport; embryonic development; intracellular transport; fibroblast growth factor receptor signaling pathway; regulation of protein localization; metabolic process; Golgi to endosome transport; Rab protein signal transduction; endocytic recycling