Rabbit RAB8B Polyclonal Antibody | anti-RAB8B antibody

RAB8B Polyclonal Antibody

Cat. Num. AAA3006470

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: RAB8B; Polyclonal Antibody; RAB8B Polyclonal Antibody; Ras-related protein Rab-8B; anti-RAB8B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RAB8B polyclonal antibody detects endogenous levels of RAB8B protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 207

• Applicable Applications for anti-RAB8B antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human RAB8B.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of RAB8B polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate)

Related Product Information for anti-RAB8B antibody

The Ras-related superfamily of guanine nucleotide binding proteins, which includes the R-Ras, Rap, Ral/Rec and Rho/Rab superfamilies exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. Rab 8B (Ras-related protein Rab-8B) is a 207 amino acid lipid anchor that localizes to the cytoplasmic side of the cell membrane and belongs to the Rab family and small GTPase superfamily. Rab 8B may be involved in neurotransmitter release and vesicular trafficking, and is encoded by a gene located on human chromosome Rab 8B and mouse chromosome 9 C.

NCBI and Uniprot Product Information

• NCBI GI #: 7706563
• NCBI GeneID: 51762
• NCBI Accession #: NP_057614.1
• NCBI GenBank Nucleotide #: NP_057614.1
• UniProt Accession #: Q92930
• Molecular Weight: ~ 42kDa
• NCBI Official Full Name: ras-related protein Rab-8B
• NCBI Official Synonym Full Names: RAB8B, member RAS oncogene family
• NCBI Official Symbol: RAB8B
• NCBI Protein Information: ras-related protein Rab-8B
• UniProt Protein Name: Ras-related protein Rab-8B
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB8B
• UniProt Entry Name: RAB8B_HUMAN

NCBI Description

RAB proteins, like RAB8B, are low molecular mass monomeric GTPases that localize on the cytoplasmic surfaces of distinct membrane-bound organelles. RAB proteins function in intracellular vesicle transport by aiding in the docking and/or fusion of vesicles with their target membranes (summary by Chen et al., 1997 [PubMed 9030196]).[supplied by OMIM, Nov 2010]

Uniprot Description

RAB8B: May be involved in vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein; Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 15q22.2

Cellular Component: peroxisomal membrane; phagocytic vesicle membrane; trans-Golgi network transport vesicle; synaptic vesicle; mitochondrion; perinuclear region of cytoplasm; secretory granule membrane; plasma membrane; phagocytic vesicle; endosome

Molecular Function: GTPase activity; protein binding; GDP binding; GTP binding; TPR domain binding; receptor binding

Biological Process: regulation of exocytosis; antigen processing and presentation; synaptic vesicle exocytosis; Golgi vesicle fusion to target membrane; cellular response to insulin stimulus; metabolic process; positive regulation of cell projection organization and biogenesis; protein secretion; cilium biogenesis; protein import into peroxisome membrane; Rab protein signal transduction; vesicle docking during exocytosis; positive regulation of adrenocorticotropic hormone secretion