Goat Rab14 Polyclonal Antibody | anti-Rab14 antibody

Rab14 Polyclonal Antibody

Cat. Num. AAA448016

• Gene Names: RAB14; FBP; RAB-14
• Reactivity: Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian
• Applications: Immunofluorescence, Western Blot
• Synonyms: Rab14; Polyclonal Antibody; Rab14 Polyclonal Antibody; Anti-Rab14; F protein-binding protein 1; bA165P4.3 (member RAS oncogene family); ras-related protein Rab-14; small GTP binding protein Rab14 antibody); anti-Rab14 antibody

• Host: Goat
• Reactivity: Human, mouse, rat, bovine, canine, chicken/avian, donkey, feline, goat, guinea pig, hamster, horse, porcine, rabbit, sheep, simian
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects Rab14 by Western blot in transfected cells with GFP-Rab14.
• Form/Format: Polyclonal antibody supplied as (2 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide. This antibody is epitope-affinity purified from goat antiserum.
• Sequence Length: 215

• Applicable Applications for anti-Rab14 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Western Blot: 1:250-1:2,000
• Gene Identifier: ENSG00000119396
• Immunogen: Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab14 produced in E. coli.
• Antigen: Purified recombinant peptide derived from within residues 110 aa to the C-terminus of mouse Rab14 produced in E. coli.
• Preparation and Storage: For continuous use, store at 2-8 deg;C for one-two days. For extended storage, store in -20 deg;C freezer. Working dilution samples should be discarded if not used within 12 hours.

Reactivity Chart

Testing Data

Related Product Information for anti-Rab14 antibody

RAB14, member RAS oncogene family

RAB14 belongs to the large RAB family of low molecular weight GTPases that are involved in intracellular membrane trafficking. This protein is expressed at high levels in kidney, lung, brain, spleen and thymus and it is thought to be involved in vesicular trafficking and neurotransmitter release. It is also involved in the biosynthetic/recycling pathway between the Golgi and endosomal compartments.

NCBI and Uniprot Product Information

• NCBI GI #: 48146905
• NCBI GeneID: 51552
• UniProt Accession #: P61106; P35287; Q5JVD4; Q6Q7K5; Q969L0; Q9UI11; B3KR31
• Molecular Weight: 23,897 Da
• NCBI Official Full Name: RAB14
• NCBI Official Synonym Full Names: RAB14, member RAS oncogene family
• NCBI Official Symbol: RAB14
• NCBI Official Synonym Symbols: FBP; RAB-14
• NCBI Protein Information: ras-related protein Rab-14; F protein-binding protein 1; small GTP binding protein RAB14; bA165P4.3 (member RAS oncogene family)
• UniProt Protein Name: Ras-related protein Rab-14
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB14
• UniProt Entry Name: RAB14_HUMAN

NCBI Description

RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM, Mar 2009]

Uniprot Description

Function: Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes

By similarity. Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion. Ref.17

Subunit structure: Interacts with KIF16B

By similarity. Interacts with ZFYVE20. Ref.18

Subcellular location: Recycling endosome. Early endosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side

By similarity. Golgi apparatus › trans-Golgi network membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle › phagosome. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side

By similarity. Note: Recruited to recycling endosomes by DENND6A. Recruited to phagosomes containing S.aureus or M.tuberculosis. Ref.13 Ref.16 Ref.17

Sequence similarities: Belongs to the small GTPase superfamily. Rab family.

Sequence caution: The sequence CAI12361.1 differs from that shown. Reason: Erroneous gene model prediction.