Western Blot (WB)
(Anti-PUMA antibody, MBS175570, Western blottingAll lanes: Anti PUMA(MBS175570) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: Rat Kidney Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD )
Rabbit PUMA Polyclonal Antibody | anti-BBC3 antibody
Anti-PUMA antibody
Western Blot (WB)
(Anti-PUMA antibody, MBS175570, Western blottingAll lanes: Anti PUMA(MBS175570) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: Rat Kidney Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD )
Western Blot (WB)
(Anti-PUMA antibody, MBS175570, Western blottingAll lanes: Anti PUMA(MBS175570) at 0.5ug/mlLane 1: HELA Whole Cell Lysate at 40ugLane 2: Rat Kidney Tissue Lysate at 50ugPredicted bind size: 21KDObserved bind size: 21KD )
Background: The p53 upregulated modulator of apoptosis, or PUMA, is a pro-apoptotic member of the Bcl-2 protein family. The PUMA gene is located at 19q. PUMA transcript is contained within 4 exons, with the presumptive initiation codon in exon 2. The predicted 193-amino acid PUMA protein shares 91% amino acid identity with the murine sequence. Bcl-2 family members can form hetero- or homodimers, and they act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The expression of PUMA is regulated by the tumor suppressor p53, and PUMA has been shown to be involved in p53-mediated apoptosis. Additionally, PUMA encodes 2 BH3 domain-containing proteins, PUMA-alpha and PUMA-beta, that are produced through the use of an alternative first exon and are induced in cells following p53 activation. Furthermore, PUMA couples the nuclear and cytoplasmic proapoptotic functions of p53.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]
Uniprot Description
PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial
Chromosomal Location of Human Ortholog: 19q13.3-q13.4
Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol
Molecular Function: protein binding
Biological Process: caspase activation; release of cytochrome c from mitochondria; apoptosis; positive regulation of protein homooligomerization; release of sequestered calcium ion into cytosol; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; determination of adult life span; response to DNA damage stimulus; negative regulation of growth
Research Articles on BBC3
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Product Notes
The BBC3 bbc3 (Catalog #AAA175570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PUMA antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PUMA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the BBC3 bbc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PUMA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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