PUMA/BBC3 protein Active Protein | PUMA/BBC3 active protein

Recombinant Human PUMA/BBC3 protein (His Tag)

Cat. Num. AAA2569249

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Purity: >90% as determined by reducing SDS-PAGE
• Synonyms: PUMA/BBC3 protein; Recombinant Human PUMA/BBC3 protein (His Tag); BBC 3; Bbc3; BBC3_HUMAN; BCL 2 binding component 3; Bcl-2-binding component 3; BCL2 binding component 3; JFY 1; JFY-1; JFY1; p53 up regulated modulator of apoptosis; p53 up-regulated modulator of apoptosis; p53 Upregulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1; PUMA/BBC3 active protein

• Host: E Coli
• Purity/Purification: >90% as determined by reducing SDS-PAGE
• Form/Format: Supplied as sterile Tris,EDTA, pH8.0.
• Sequence: 1M-193N

• Species: Human
• Tag: N-His
• Endotoxin: Not tested.
• Protein Construction: A DNA sequence encoding the Human PUMA/BBC3 (Q9BXH1-1)(1M-193N) was expressed with a polyhistidine tag at the N-terminus.
• Activity: Immunogen (E-AB-40332)
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C.Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt, store it immediately at<-20 degree C.

SDS-Page

Related Product Information for PUMA/BBC3 active protein

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 56748610
• NCBI GeneID: 27113
• UniProt Accession #: Q9BXH1; O00171; Q96PG9; B9EGI3; Q96PG8
• Molecular Weight: 193
• NCBI Official Full Name: Bcl-2-binding component 3
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3; p53 up-regulated modulator of apoptosis
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of neuron apoptosis; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth