Rabbit anti-Human, Mouse PUMA Polyclonal Antibody | anti-PUMA antibody

PUMA Antibody

Cat. Num. AAA9400872

• Gene Names: BBC3; JFY1; PUMA; JFY-1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Affinity chromatography purified via peptide column
• Synonyms: PUMA; Polyclonal Antibody; PUMA Antibody; bbc3; anti-PUMA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 193

• Applicable Applications for anti-PUMA antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a synthetic peptide corresponding to 14 amino acids near the carboxy terminus of human PUMA-a. This sequence is identical between a and b forms of the PUMA proteins.
• Target Name: PUMA
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lysates with PUMA antibody at 2 ug/ml)

Immunocytochemistry (ICC)

(Immunocytochemistry of PUMA in K562 cells with PUMA antibody at 1 ug/ml.)

Related Product Information for anti-PUMA antibody

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse. PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.

Product Categories/Family for anti-PUMA antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 15193488
• NCBI GeneID: 27113
• NCBI Accession #: NP_055232
• NCBI GenBank Nucleotide #: NM_014417.4
• Molecular Weight: 10,326 Da
• NCBI Official Full Name: bcl-2-binding component 3 isoform 4
• NCBI Official Synonym Full Names: BCL2 binding component 3
• NCBI Official Symbol: BBC3
• NCBI Official Synonym Symbols: JFY1; PUMA; JFY-1
• NCBI Protein Information: bcl-2-binding component 3
• UniProt Protein Name: Bcl-2-binding component 3
• UniProt Gene Name: BBC3
• UniProt Synonym Gene Names: PUMA
• UniProt Entry Name: BBC3_HUMAN

NCBI Description

This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]

Uniprot Description

PUMA: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Interacts with MCL1 and BCL2A1. Interacts with BCL2 and BCL2L1/BCL-XL. By DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. Ubiquitously expressed. Belongs to the Bcl-2 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: mitochondrial outer membrane; mitochondrion; cytosol

Molecular Function: protein binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of protein homooligomerization; apoptosis; positive regulation of neuron apoptosis; reduction of endoplasmic reticulum calcium ion concentration; release of sequestered calcium ion into cytosol; determination of adult life span; response to DNA damage stimulus; negative regulation of growth