Rabbit AMP Activated Protein Kinase gamma2 Polyclonal Antibody | anti-PRKAG2 antibody

AMP Activated Protein Kinase gamma2 (AMPK g2)

Cat. Num. AAA613726

• Gene Names: PRKAG2; AAKG; CMH6; WPWS; AAKG2; H91620p
• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Synonyms: AMP Activated Protein Kinase gamma2; Polyclonal Antibody; AMP Activated Protein Kinase gamma2 (AMPK g2); Anti -AMP Activated Protein Kinase gamma2 (AMPK g2); anti-PRKAG2 antibody

• Host: Rabbit
• Reactivity: Bovine, Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human AMPKg2 protein at ~75kD. Species Crossreactivity: mouse, rat, monkey and bovine.
• Purity/Purification: Affinity Purified; Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-PRKAG2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide correspoding to residues surrounding Ser60 of human AMPKg2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot. Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from 293 (human), NBT-II (rat), and Neuro-2A (mouse) cells, using MBS613726.)

Related Product Information for anti-PRKAG2 antibody

AMP-activated protein kinase (AMPK) is highly conserved from yeast to plants and animals and plays a key role in the regulation of energy homeostasis 1). AMPK is a heterotrimeric complex composed of a catalytic a subunit and regulatory b and g subunits, each of which is encoded by two or three distinct genes a1, 2; b1, 2; g1, 2, 3) (2). The kinase is activated by an elevated AMP/ATP ratio due to cellular and environmental stress, such as heat shock, hypoxia and ischemia (1). Tumor suppressor LKB1, in association with accessory proteins STRAD and MO25, phosphorylates AMPK at Thr172 at the activation loop, and this phosphorylation is required for AMPK activation (3-5). AMPKa is also phosphorylated at Thr258 and Ser485 for a1; Ser491 for a2). The upstream kinase and the biological significance of these phosphorylation events have yet to be elucidated (6). The b1 subunit is posttranslationally modified by myristoylation and multi-site phosphorylation including Ser24/25, Ser96, Ser101, Ser108, and Ser182 (6,7). Phosphorylation at Ser108 of the b1 subunit seems to be required for the activation of AMPK enzyme, while phosphorylation at Ser24/25 and Ser182 affects AMPK localization (7). Several mutations in AMPKg subunits have been identified, most of which are located in the putative AMP/ATP binding sites (CBS or Bateman domains). Mutations at these sites lead to reduction of AMPK activity and cause glycogen accumulation in heart or skeletal muscle (1,2). Accumulating evidence indicates that AMPK not only regulates the metabolism of fatty acids and glycogen, but also modulates protein synthesis and cell growth through EF2 and TSC2/mTOR pathways, as well as blood flow via eNOS/nNOS (1).

Product Categories/Family for anti-PRKAG2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6688199
• NCBI GeneID: 51422
• UniProt Accession #: Q9UGJ0; Q53Y07; Q6NUI0; Q75MP4; Q9NUZ9; Q9UDN8; Q9ULX8; Q8NCK6
• Molecular Weight: 63,066 Da
• NCBI Official Full Name: AMP-activated protein kinase gamma2 subunit
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• NCBI Official Symbol: PRKAG2
• NCBI Official Synonym Symbols: AAKG; CMH6; WPWS; AAKG2; H91620p
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; OTTHUMP00000211953; OTTHUMP00000211954; OTTHUMP00000211955; OTTHUMP00000211956; AMPK subunit gamma-2
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAG2
• UniProt Entry Name: AAKG2_HUMAN

NCBI Description

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family and encodes a protein with four cystathionine beta-synthase domains. Mutations in this gene have been associated with ventricular pre-excitation (Wolff-Parkinson-White syndrome), progressive conduction system disease and cardiac hypertrophy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Uniprot Description

AMPKG2: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non- catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Isoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis. Belongs to the 5'-AMP-activated protein kinase gamma subunit family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Autophagy; Protein kinase, regulatory subunit

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: nucleoplasm; extracellular space; cytosol; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; cAMP-dependent protein kinase inhibitor activity; cAMP-dependent protein kinase regulator activity; phosphorylase kinase regulator activity; ADP binding; protein kinase binding; protein kinase activator activity; ATP binding; AMP binding

Biological Process: glycogen metabolic process; mitochondrion organization and biogenesis; organelle organization and biogenesis; cellular lipid metabolic process; carnitine shuttle; protein amino acid phosphorylation; regulation of fatty acid biosynthetic process; regulation of glycolysis; sterol biosynthetic process; positive regulation of protein kinase activity; ATP biosynthetic process; regulation of fatty acid metabolic process; regulation of fatty acid oxidation; insulin receptor signaling pathway; energy reserve metabolic process; negative regulation of protein kinase activity; regulation of glucose import; cell cycle arrest; fatty acid biosynthetic process

Disease: Glycogen Storage Disease Of Heart, Lethal Congenital; Cardiomyopathy, Familial Hypertrophic, 6; Wolff-parkinson-white Syndrome