Rabbit anti-Human PSMD7 Polyclonal Antibody | anti-PSMD7 antibody

Anti-PSMD7 Antibody, Rabbit Polyclonal

Cat. Num. AAA8108088

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD7; Polyclonal Antibody; Anti-PSMD7 Antibody; Rabbit Polyclonal; PSMD7 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome 26S subunit; non-ATPase 7; Anti-MOV34 Antibody; Anti-P40 Antibody; Anti-Rpn8 Antibody; Anti-S12 Antibody; anti-PSMD7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD7
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD7 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:200-1:1000; IP: 1-5uL/mg of lysate
• Immunogen: E Coli-derived Human PSMD7 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human PSMD7 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMD7 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: MCF7 Whole Cell Lysate Lane C: A549 Whole Cell Lysate Lane D: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:37 kDa Observed band size:41 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human PSMD7 in human kidney with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(PSMD7 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-PSMD7 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMD7 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa Observed band size :43 kDa)

Related Product Information for anti-PSMD7 antibody

PSMD7 (Proteasome 26S Subunit, Non-ATPase 7) is a Protein Coding gene. The PSMD7 gene, located on 16q23.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 17. PSMD7, a 19S proteasome subunit, is overexpressed in most carcinoma cells. It forms a dimer with PSMD14 that functions in the removal of the attached ubiquitin chain. PSMD7 is widely expressed in bone marrow, placenta, and other tissues. Diseases associated with PSMD7 include Huntington's Disease and Tracheitis. PSMD7 and the mTOR/p70S6K pathway may be a promising candidate for developing therapies for esophageal squamous cell carcinoma (ESCC).

References

Shi K, et al. (2018) Psmd7 downregulation induces apoptosis and suppresses tumorigenesis of esophageal squamous cell carcinoma via the mtor/p70s6k pathway. FEBS Open Bio 8 (4): 533-543.Niu Z, et al. (2011) A polymorphism rs17336700 in the psmd7 gene is associated with ankylosing spondylitis in chinese subjects. Ann Rheum Dis 70 (4): 706-707.Alves BS, et al. (2006) Characterization of the human ortholog of mov34 reveals eight n-terminal residues important for mpn domain stability. Biochem Biophys Res Commun 347 (3): 608-615.