Rabbit PSMD2 Polyclonal Antibody | anti-PSMD2 antibody

Anti-PSMD2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8104031

• Gene Names: PSMD2; S2; P97; Rpn1; TRAP2
• Reactivity: Human; Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: PSMD2; Polyclonal Antibody; Anti-PSMD2 Antibody; Rabbit Polyclonal; PSMD2 Antibody; Rabbit PAb; Antigen Affinity Purified; proteasome (prosome; macropain) 26S subunit; non-ATPase; 2; Anti-P97 Antibody; Anti-RPN1 Antibody; Anti-S2 Antibody; Anti-TRAP2 Antibody; anti-PSMD2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Pongo abelii (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PSMD2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-PSMD2 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human PSMD2
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PSMD2, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-PSMD2 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:100 kDa Observed band size:100 kDa)

Immunoprecipitation (IP)

(PSMD2 was immunoprecipitated using: Lane A:0.5 mg Jurkat Whole Cell Lysate 4 uL anti-PSMD2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-PSMD2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 100 kDa Observed band size :100 kDa)

Related Product Information for anti-PSMD2 antibody

PSMD2 (Proteasome 26S Subunit, Non-ATPase 2) is a Protein Coding gene. The PSMD2 gene, located on 3q27.1, is conserved in chimpanzee, Rhesus monkey, dog, cow, mouse, and rat, etc. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signaling pathway since it interacts with the tumor necrosis factor type 1 receptor. PSMD2 is widely expressed in the testis, brain, and other tissues. Diseases associated with PSMD2 include Chapare Hemorrhagic Fever and Cystic Fibrosis. PSMD2 is a constituent of a signature associated with the acquisition of metastatic phenotype and poor prognosis in lung cancers. The related pathways of PSMD2 are Regulation of activated PAK-2p34 by proteasome-mediated degradation and Antigen processing-Cross presentation.

References

Tsurumi C, et al. (1996) Cdna cloning and functional analysis of the p97 subunit of the 26s proteasome, a polypeptide identical to the type-1 tumor-necrosis-factor-receptor-associated protein-2/55.11. Eur J Biochem 239 (3): 912-921.Tan Y, et al. (2019) Psmd1 and psmd2 regulate hepg2 cell proliferation and apoptosis via modulating cellular lipid droplet metabolism. BMC Mol Biol 20 (1): 24.Matsuyama Y, et al. (2011) Proteasomal non-catalytic subunit psmd2 as a potential therapeutic target in association with various clinicopathologic features in lung adenocarcinomas. Mol Carcinog 50 (4): 301-309.

NCBI and Uniprot Product Information

• NCBI GI #: 25777602
• NCBI GeneID: 5708
• NCBI Accession #: NP_002799.3
• NCBI GenBank Nucleotide #: NM_002808.3
• UniProt Accession #: Q13200; Q12932; Q15321; Q53XQ4; Q96I12
• Molecular Weight: 100,200 Da
• NCBI Official Full Name: 26S proteasome non-ATPase regulatory subunit 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) 26S subunit, non-ATPase, 2
• NCBI Official Symbol: PSMD2
• NCBI Official Synonym Symbols: S2; P97; Rpn1; TRAP2
• NCBI Protein Information: 26S proteasome non-ATPase regulatory subunit 2; 55.11 protein; protein 55.11; TNFR-associated protein 2; 26S proteasome subunit p97; 26S proteasome regulatory subunit S2; 26S proteasome regulatory subunit RPN1; tumor necrosis factor receptor-associated pr
• UniProt Protein Name: 26S proteasome non-ATPase regulatory subunit 2
• Protein Family: 26S proteasome non-ATPase regulatory
• UniProt Gene Name: PSMD2
• UniProt Synonym Gene Names: TRAP2
• UniProt Entry Name: PSMD2_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1. [provided by RefSeq, Jul 2008]

Uniprot Description

PSMD2: Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. Belongs to the proteasome subunit S2 family.

Protein type: Cell cycle regulation; Proteasome complex

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: proteasome complex; nucleoplasm; membrane; proteasome regulatory particle; cytosol

Molecular Function: protein binding; enzyme regulator activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle