Rabbit Protein Synthesis Initiation Factor 4E Polyclonal Antibody

Protein Synthesis Initiation Factor 4E (eIF 4E, Eukaryotic Translation Initiation Factor)

Cat. Num. AAA615503

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Protein Synthesis Initiation Factor 4E; Polyclonal Antibody; Protein Synthesis Initiation Factor 4E (eIF 4E; Eukaryotic Translation Initiation Factor); Anti -Protein Synthesis Initiation Factor 4E (eIF 4E; anti-Protein Synthesis Initiation Factor 4E antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes endogenous levels of total human eIF4E protein. The antibody does not cross-react with other proteins. Species Crossreactivity: mouse, rat, monkey and zebra fish.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-Protein Synthesis Initiation Factor 4E antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistchemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Paraffin and Frozen): 1:100
• Immunogen: Synthetic peptide corresponding to residues around Ser209 of eIF4E (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from control and serum treated NIH/3T3, 293 or PC12 cells, using MBS615503.)

Related Product Information for anti-Protein Synthesis Initiation Factor 4E antibody

Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure thereby mediating the initiation of translation (1,2). eIF4E interacts with eIF4G, which serves as a scaffold protein for the assembly of eIF4E and eIF4A to form the eIF4F complex (2). Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 has been shown to phosphorylate eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites have been identified in mouse Mnk1, Thr197 and Thr202, which are essential for Mnk1 kinase activity (3). The C-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148 and Ser1192 (5). It is known that their phosphorylation is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin.

Product Categories/Family for anti-Protein Synthesis Initiation Factor 4E antibody

Antibodies; Abs to Translation Initiation Factors

NCBI and Uniprot Product Information

• NCBI GI #: 171446
• NCBI Official Full Name: protein synthesis initiation factor eIF-4E

Uniprot Description

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.