Rabbit anti-Human CPSF3 Polyclonal Antibody | anti-CPSF3 antibody

CPSF3, ID (CPSF3, CPSF73, Cleavage and polyadenylation specificity factor subunit 3, Cleavage and polyadenylation specificity factor 73kD subunit, mRNA 3'-end-processing endonuclease CPSF-73) (Azide free) (HRP)

Cat. Num. AAA6288410

• Gene Names: CPSF3; CPSF73; CPSF-73
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPSF3; Polyclonal Antibody; ID (CPSF3; CPSF73; Cleavage and polyadenylation specificity factor subunit 3; Cleavage and polyadenylation specificity factor 73kD subunit; mRNA 3'-end-processing endonuclease CPSF-73) (Azide free) (HRP); mRNA 3'-end-processing endonuclease CPSF-73; anti-CPSF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-CPSF3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPSF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 191-221 amino acids from the Central region of human CPSF3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CPSF3 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CPSF3 antibody detected the CPSF3 protein (arrow).)

Related Product Information for anti-CPSF3 antibody

Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al., 2006 [PubMed 17128255]).

Product Categories/Family for anti-CPSF3 antibody

Antibodies; Enzymes; Endonuclease

References

Rozenblatt-Rosen, O., et al. Proc. Natl. Acad. Sci. U.S.A. 106(3):755-760(2009). Zhu, Z.H., et al. Oncogene 28(1):41-51(2009). Yang, X.C., et al. Mol. Cell. Biol. 29(1):31-42(2009). Kolev, N.G., et al. EMBO Rep. 9(10):1013-1018(2008). de la Vega, L., et al. J. Mol. Biol. 372(2):317-330(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 7706427
• NCBI GeneID: 51692
• NCBI Accession #: NP_057291.1
• NCBI GenBank Nucleotide #: NM_016207.3
• UniProt Accession #: Q9UKF6; O14769; Q53RS2; Q96F36
• Molecular Weight: 77,486 Da
• NCBI Official Full Name: cleavage and polyadenylation specificity factor subunit 3
• NCBI Official Synonym Full Names: cleavage and polyadenylation specific factor 3, 73kDa
• NCBI Official Symbol: CPSF3
• NCBI Official Synonym Symbols: CPSF73; CPSF-73
• NCBI Protein Information: cleavage and polyadenylation specificity factor subunit 3; mRNA 3'-end-processing endonuclease CPSF-73
• UniProt Protein Name: Cleavage and polyadenylation specificity factor subunit 3
• Protein Family: Cleavage and polyadenylation specificity factor
• UniProt Gene Name: CPSF3
• UniProt Synonym Gene Names: CPSF73; CPSF 73 kDa subunit
• UniProt Entry Name: CPSF3_HUMAN