Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4E antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Rabbit EIF4E Polyclonal Antibody | anti-EIF4E antibody
EIF4E Polyclonal Antibody
IHC: 1:50 - 1:200
ICC: 1:50 - 1:200
IP: 1:50 - 1:100
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4E antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EIF4E antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody at dilution of 1:100 (40x lens).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
Uniprot Description
EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.
Protein type: Translation; Translation initiation
Chromosomal Location of Human Ortholog: 4q23
Cellular Component: mRNA cap complex; stress granule; eukaryotic translation initiation factor 4F complex; cytoplasm; cytosol
Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding
Biological Process: viral reproduction; translation; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; translational initiation; insulin receptor signaling pathway; gene expression; G1/S transition of mitotic cell cycle; lung development; mRNA catabolic process, deadenylation-dependent decay
Disease: Autism, Susceptibility To, 19
Research Articles on EIF4E
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Product Notes
The EIF4E eif4e (Catalog #AAA126936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EIF4E Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's EIF4E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 ICC: 1:50 - 1:200 IP: 1:50 - 1:100. Researchers should empirically determine the suitability of the EIF4E eif4e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF4E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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