Western Blot (WB)
((2ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat HSD17B3 Polyclonal Antibody | anti-HSD17B3 antibody
Goat anti-HSD17B3 (aa141-155) Antibody
Western Blot: Approx 33kDa band observed in Human Testis lysates (calculated MW of 34.5kDa according to NP_000188.1). Recommended concentration: 1-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
Uniprot Description
HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
Protein type: EC 1.1.1.64; Lipid Metabolism - androgen and estrogen; Oxidoreductase
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; intracellular membrane-bound organelle
Molecular Function: 3-alpha(17-beta)-hydroxysteroid dehydrogenase (NAD+) activity; testosterone 17-beta-dehydrogenase (NADP+) activity
Biological Process: androgen biosynthetic process; male genitalia development
Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
Research Articles on HSD17B3
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Product Notes
The HSD17B3 hsd17b3 (Catalog #AAA423643) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HSD17B3 (aa141-155) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 33kDa band observed in Human Testis lysates (calculated MW of 34.5kDa according to NP_000188.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the HSD17B3 hsd17b3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SHFLNAPDEI QSLIH. It is sometimes possible for the material contained within the vial of "HSD17B3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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