Western Blot (WB)
((2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat B56 beta isoform Polyclonal Antibody | anti-PPP2R5B antibody
Goat anti-B56 beta isoform Antibody
Western Blot: Approx.55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 57.4kDa according to NP_006235.1). Recommended concentration: 2-3ug/ml.
Western Blot (WB)
((2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((2ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq, Jul 2008]
Uniprot Description
PPP2R5B: a brain-specific, B regulatory subunit of the PP2A phosphoprotein phosphatase that modulates substrate selectivity, localization, and catalytic activity of the holoenzyme. Targeted by the KLHL15-Cul3 ubiquitin ligase complex, promoting its turnover by ubiquitylation and proteasomal degradation. Mediates dephosphorylation of tyrosine hydroxylase (TH) and calcium/calmodulin-dependent protein kinase type II (CaMK2). The phosphorylated form mediates the interaction between AKT1 and PP2A phosphatase leading to dephosphorylation of AKT1 pT308 and pS373. Belongs to the phosphatase 2A regulatory subunit B56 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Protein phosphatase, regulatory subunit
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: cytoplasm; cytosol; protein phosphatase type 2A complex
Molecular Function: protein binding; protein phosphatase type 2A regulator activity
Biological Process: positive regulation of nerve growth factor receptor signaling pathway; positive regulation of protein complex assembly; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of peptidyl-tyrosine phosphorylation; regulation of phosphoinositide 3-kinase cascade; regulation of protein amino acid autophosphorylation; regulation of protein phosphatase type 2A activity; regulation of receptor activity
Research Articles on PPP2R5B
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Product Notes
The PPP2R5B ppp2r5b (Catalog #AAA421373) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-B56 beta isoform Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's B56 beta isoform can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 2000. Western Blot: Approx.55kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 57.4kDa according to NP_006235.1). Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the PPP2R5B ppp2r5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QRLTPQVAAS GGQS. It is sometimes possible for the material contained within the vial of "B56 beta isoform, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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