Rabbit BID Polyclonal Antibody | anti-BID antibody

BID Antibody

Cat. Num. AAA2518634

• Gene Names: BID; FP497
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: BID; Polyclonal Antibody; BID Antibody; FP497; MGC15319; MGC42355; anti-BID antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 195

• Applicable Applications for anti-BID antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Recombinant protein of human BID
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6539 / sc-6538 / sc-11423
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of HL60 cell lines, using BID antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat brain using BID antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human lung cancer using BID antibody at dilution of 1:200 (200x lens).)

Related Product Information for anti-BID antibody

The BH3 domain-only protein, BID, a death agonist member of the Bcl-2/Bcl-xL family (1), is localized in the cytosolic fraction of cells as an inactive precursor (2, 3). Its active form is generated upon proteolytic cleavage by caspase-8 in the Fas signaling pathway. Cleaved BID translocates to mitochondria and induces cytochrome c release and mitochondrial damage (2-5). Thus, BID relays an apoptotic signal from the cell surface to mitochondria. However, the precise molecular mechanism for the translocation of the cleaved BID, and for the subsequent release of cytochrome c during apoptosis, is still unclear.

NCBI and Uniprot Product Information

• NCBI GI #: 2493285
• NCBI GeneID: 637
• Molecular Weight: 11,263 Da
• NCBI Official Full Name: BH3-interacting domain death agonist
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; Human BID coding sequence; apoptic death agonist; desmocollin type 4; p22 BID
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Mitochondrial; Apoptosis

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; neuron apoptosis; apoptotic mitochondrial changes; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization