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Western Blot (WB) ((2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Goat HOXD12 Polyclonal Antibody | anti-HOXD12 antibody

Goat anti-HOXD12 Antibody

Gene Names
HOXD12; HOX4H
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
HOXD12; Polyclonal Antibody; Goat anti-HOXD12 Antibody; homeobox D12; HOX4H; Hox-4.7; mouse; homolog of; homeo box D12; homeobox protein Hox-4H; homeobox protein Hox-D12; HOXD12 antibody; homeobox D12 antibody; HOX4H antibody; homolog of antibody; homeo box D12 antibody; homeobox protein Hox-4H antibody; homeobox protein Hox-D12 antibody; anti-HOXD12 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
PTAKDGPEEQAK
Sequence Length
270
Applicable Applications for anti-HOXD12 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 1000.
Western Blot: Approx 37kDa band observed in Human Heart lysates (calculated MW of 29. 0kDa according to NP_067016.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-3ug/ml.
Immunogen
Peptide with sequence C-PTAKDGPEEQAK, from the internal region of the protein sequence according to NP_067016.3.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.

Western Blot (WB)

((2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Western Blot (WB) ((2ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,304 Da
NCBI Official Full Name
homeobox protein Hox-D12
NCBI Official Synonym Full Names
homeobox D12
NCBI Official Symbol
HOXD12
NCBI Official Synonym Symbols
HOX4H
NCBI Protein Information
homeobox protein Hox-D12
UniProt Protein Name
Homeobox protein Hox-D12
Protein Family
UniProt Gene Name
HOXD12
UniProt Synonym Gene Names
HOX4H
UniProt Entry Name
HXD12_HUMAN

NCBI Description

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. The exact role of this gene has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

HOXD12: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Belongs to the Abd-B homeobox family.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: nucleus; transcription factor complex

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: embryonic digit morphogenesis; pattern specification process; regulation of transcription, DNA-dependent; skeletal development; transcription, DNA-dependent

Research Articles on HOXD12

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Product Notes

The HOXD12 hoxd12 (Catalog #AAA423042) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-HOXD12 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HOXD12 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 1000. Western Blot: Approx 37kDa band observed in Human Heart lysates (calculated MW of 29. 0kDa according to NP_067016.3). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 2-3ug/ml. Researchers should empirically determine the suitability of the HOXD12 hoxd12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: PTAKDGPEEQ AK. It is sometimes possible for the material contained within the vial of "HOXD12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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