Western Blot (WB)
(Western Blot (WB) analysis of PIASy (D488) pAb at 1:1000 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:K562 whole cell lysate (10ug))
Rabbit PIASy Polyclonal Antibody | anti-PIASy antibody
PIASy (D488) Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
PIASy; Polyclonal Antibody; PIASy (D488) Polyclonal Antibody; E3 SUMO-protein ligase PIAS4; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; PIAS-gamma; PIAS4; PIASG; anti-PIASy antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PIAS4 (D488) polyclonal antibody detects endogenous levels of PIAS4 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Applicable Applications for anti-PIASy antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
Synthetic peptide, corresponding to amino acids 455-506 of Human PIASy.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of PIASy (D488) pAb at 1:1000 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:K562 whole cell lysate (10ug))
Western Blot (WB)
(Western Blot (WB) analysis of PIASy (D488) pAb at 1:1000 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:MEF whole cell lysate (40ug) Lane3:The Testis tissue lysate of Rat (20ug) Lane4:HEK293T whole cell lysate (10ug) Lane5:K562 whole cell lysate (10ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of PIASy (D488) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of PIASy (D488) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)
Related Product Information for anti-PIASy antibody
The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.
NCBI and Uniprot Product Information
UniProt Accession #
Molecular Weight
~ 56kDa
Similar Products
Product Notes
The PIASy (Catalog #AAA3001131) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PIASy (D488) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PIASy can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the PIASy for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIASy, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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