Rabbit anti-Human, Mouse NIPA Polyclonal Antibody | anti-NIPA antibody

NIPA (S350) Polyclonal Antibody

Cat. Num. AAA3001760

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NIPA; Polyclonal Antibody; NIPA (S350) Polyclonal Antibody; Nuclear-interacting partner of ALK; Nuclear-interacting partner of anaplastic lymphoma kinase; hNIPA; Zinc finger C3HC-type protein 1; ZC3HC1; HSPC216; anti-NIPA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NIPA (S350) polyclonal antibody detects endogenous levels of NIPA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 502

• Applicable Applications for anti-NIPA antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 318-372 of Human NIPA.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NIPA (S350) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (20ug) Lane2:K562 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (10ug) Lane4:AML-12 whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of NIPA (S350) pAb at 1:500 dillution Lane1:HEK293T whole cell lysateLane2:U-87MG whole cell lysateLane3:H9C2 whole cell lysateLane4:CT-26 whole cell lysateLane5:The Testis tissue lysate of Mouse Lane6:The Testis tissue lysate of Rat)

Related Product Information for anti-NIPA antibody

Entry into mitosis is essentially driven by cyclin B1 which is located in the cytoplasm throughout interphase, but accumulates in the nucleus just before mitosis occurs. Nuclear Interaction Partner of ALK (NIPA) plays a critical role in cyclin B1 regulation. NIPA is normally phosphorylated during G2 and M phases, resulting in an accumulation of cyclin B1. When NIPA sheds its attached phosphate, it binds to SCF to form the SCFNIPA complex, a member of the E3 ubiquitin ligases, which ubiquitinates cyclin B1, thereby targeting it to the proteosome for degradation.

NCBI and Uniprot Product Information

• NCBI GI #: 66348045
• NCBI Accession #: NP_057562.3
• NCBI GenBank Nucleotide #: NP_057562.3
• UniProt Accession #: Q86WB0
• Molecular Weight: ~ 60-65kDa
• NCBI Official Full Name: nuclear-interacting partner of ALK isoform 1
• UniProt Protein Name: Nuclear-interacting partner of ALK
• Protein Family: NIPA-like protein
• UniProt Gene Name: ZC3HC1
• UniProt Synonym Gene Names: NIPA; hNIPA
• UniProt Entry Name: NIPA_HUMAN

Uniprot Description

NIPA: a nuclear protein that interacts with anaplastic lymphoma kinase (ALK). An essential component of an SCF-type E3 ligase complex, SCF(NIPA), a complex that controls mitotic entry by mediating ubiquitination and subsequent degradation of cyclin B1. Its cell-cycle-dependent phosphorylation regulates the assembly of the SCF(NIPA) complex, restricting cyclin B1 ubiquitination activity to interphase. Its inactivation results in nuclear accumulation of cyclin B1 in interphase and premature mitotic entry. May have an antiapoptotic role in NPM-ALK-mediated signaling events. Interacts with the NPM-ALK fusion protein in a tyrosine phosphorylation-dependent manner. Three splice-variant isoforms have been described.

Protein type: Cell cycle regulation; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 7q32.2

Cellular Component: nuclear membrane; nucleus

Molecular Function: protein binding; zinc ion binding; protein kinase binding

Biological Process: mitosis; cell division; protein ubiquitination