Western Blot (WB)
(Western blot analysis of CENPK using COLO205 whole cell lysates)
Rabbit anti-Human, Mouse CENPK Polyclonal Antibody | anti-CENPK antibody
CENPK Antibody
Gene Names
CENPK; P33; Solt; CENP-K; FKSG14; AF5alpha
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
CENPK; Polyclonal Antibody; CENPK Antibody; AF5 alpha; AF5alpha; B130045K24Rik; BB232189; C530004N04Rik; CENP K; Centromere protein K; Centromeric protein K; FKSG 14; FKSG14; ICEN 37; ICEN37; Interphase centromere complex protein 37; Leucine zipper protein FKSG14; OTTHUMP00000161888; P33; Protein AF 5alpha; Protein AF5alpha; Solt; Solzt; SoxLZ/Sox6 binding protein Solt; SoxLZ/Sox6 leucine zipper binding protein; anti-CENPK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
CENPK antibody detects endogenous levels of total CENPK
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
269
Applicable Applications for anti-CENPK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human CENPK
Subcellular Location
Nuclear. Centromere. Kinetochore. Localizes exclusively in the centromeres.
Tissue Specificity
Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of CENPK using COLO205 whole cell lysates)
Western Blot (WB)
(Western blot analysis of CENPK using COLO205 whole cell lysates)
Related Product Information for anti-CENPK antibody
Function: Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
Subunit Structure: Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts directly with CENPH.
Similarity: Belongs to the CENP-K/MCM22 family.
Subunit Structure: Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex interacts with the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU. Interacts directly with CENPH.
Similarity: Belongs to the CENP-K/MCM22 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 32 kDa
Predicted: 32 kDa
Predicted: 32 kDa
NCBI Official Full Name
centromere protein K isoform 1
NCBI Official Synonym Full Names
centromere protein K
NCBI Official Symbol
CENPK
NCBI Official Synonym Symbols
P33; Solt; CENP-K; FKSG14; AF5alpha
NCBI Protein Information
centromere protein K
UniProt Protein Name
Centromere protein K
Protein Family
UniProt Gene Name
CENPK
UniProt Synonym Gene Names
ICEN37; CENP-K
NCBI Description
CENPK is a subunit of a CENPH (MIM 605607)-CENPI (MIM 300065)-associated centromeric complex that targets CENPA (MIM 117139) to centromeres and is required for proper kinetochore function and mitotic progression (Okada et al., 2006 [PubMed 16622420]).[supplied by OMIM, Mar 2008]
Uniprot Description
Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with KNL1 to recruit the NDC80 complex to the outer kinetochore.
Research Articles on CENPK
Similar Products
Product Notes
The CENPK cenpk (Catalog #AAA9609376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CENPK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CENPK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the CENPK cenpk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CENPK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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