Rabbit PIAS3 Polyclonal Antibody | anti-PIAS3 antibody

PIAS3 (F25) Polyclonal Antibody

Cat. Num. AAA3001129

• Gene Names: PIAS3; ZMIZ5
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PIAS3; Polyclonal Antibody; PIAS3 (F25) Polyclonal Antibody; E3 SUMO-protein ligase PIAS1; Protein inhibitor of activated STAT protein 1; Gu binding protein; GBP; RNA helicase II binding protein; DEAD/H box-binding protein 1; DDXBP1; anti-PIAS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIAS3 (F25) polyclonal antibody detects endogenous levels of PIAS3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 628

• Applicable Applications for anti-PIAS3 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to the N-terminual of Human PIAS 3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PIAS 3 (F25) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Related Product Information for anti-PIAS3 antibody

The IL-6 type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization, and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

NCBI and Uniprot Product Information

• NCBI GI #: 115298686
• NCBI GeneID: 10401
• NCBI Accession #: NP_006090.2
• NCBI GenBank Nucleotide #: NP_006090.2
• UniProt Accession #: Q9Y6X2
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS3
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 3
• NCBI Official Symbol: PIAS3
• NCBI Official Synonym Symbols: ZMIZ5
• NCBI Protein Information: E3 SUMO-protein ligase PIAS3
• UniProt Protein Name: E3 SUMO-protein ligase PIAS3
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS3
• UniProt Entry Name: PIAS3_HUMAN

NCBI Description

This gene encodes a member of the PIAS [protein inhibitor of activated STAT (signal transducer and activator of transcription)] family of transcriptional modulators. The protein functions as a SUMO (small ubiquitin-like modifier)-E3 ligase which catalyzes the covalent attachment of a SUMO protein to specific target substrates. It directly binds to several transcription factors and either blocks or enhances their activity. Alternatively spliced transcript variants of this gene have been identified, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

PIAS3: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family.

Protein type: EC 6.3.2.-; SUMO conjugating system; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; dendrite; synapse; nucleus

Molecular Function: protein C-terminus binding; protein binding; enzyme binding; potassium channel regulator activity; zinc ion binding; protein N-terminus binding; SUMO ligase activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential