Rabbit PIAS 1 Polyclonal Antibody | anti-PIAS1 antibody

PIAS 1 (G24) Polyclonal Antibody

Cat. Num. AAA3001125

• Gene Names: PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PIAS 1; Polyclonal Antibody; PIAS 1 (G24) Polyclonal Antibody; E3 SUMO-protein ligase PIAS1; Protein inhibitor of activated STAT protein 1; Gu binding protein; GBP; RNA helicase II binding protein; DEAD/H box-binding protein 1; DDXBP1; anti-PIAS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIAS1 (G24) polyclonal antibody detects endogenous levels of PIAS1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 651

• Applicable Applications for anti-PIAS1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human PIAS 1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PIAS 1 (G24) polyclonal antibody at 1:500 dilutionLane1:MCF-7 whole cell lysateLane2:Raw264. 7 whole cell lysateLane3:H9C2 whole cell lysate)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of PIAS 1 (G24) pAb in paraffin-embedded human lung carcinoma tissue at 1:100.)

Western Blot (WB)

(Western Blot (WB) analysis of PIAS 1 (G24) pAb at 1:500 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:CT26 whole cell lysate of Rat (40ug) Lane4:The Testis tissue lysate of Rat (40ug))

Related Product Information for anti-PIAS1 antibody

The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

NCBI and Uniprot Product Information

• NCBI GI #: 7706637
• NCBI GeneID: 8554
• NCBI Accession #: NP_057250.1
• NCBI GenBank Nucleotide #: NP_057250.1
• UniProt Accession #: O75925
• Molecular Weight: ~ 72kDa
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS1 isoform 2
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 1
• NCBI Official Symbol: PIAS1
• NCBI Official Synonym Symbols: GBP; ZMIZ3; DDXBP1; GU/RH-II
• NCBI Protein Information: E3 SUMO-protein ligase PIAS1
• UniProt Protein Name: E3 SUMO-protein ligase PIAS1
• Protein Family: E3 SUMO-protein ligase
• UniProt Gene Name: PIAS1
• UniProt Synonym Gene Names: DDXBP1; GBP
• UniProt Entry Name: PIAS1_HUMAN

NCBI Description

This gene encodes a member of the protein inhibitor of activated STAT (PIAS) family. PIAS proteins function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. This protein plays a central role as a transcriptional coregulator of numerous cellular pathways includign the STAT1 and nuclear factor kappaB pathways. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]

Uniprot Description

PIAS1: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. Together with PRMT1, may repress STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. Interacts with NCOA2 and AR. Interacts with NR2C1; the interaction promotes its sumoylation. Interacts with DDX21, CSRP2, AXIN1, JUN, UBE2I, SUMO1, SATB2, PLAG1, TP53 and STAT1 (dimer), following IFNA1-stimulation. Interacts with SP3 (preferentially when SUMO-modified). Interacts with KLF8; the interaction results in SUMO ligation and repression of KLF8 transcriptional activity and of its cell cycle progression into G(1) phase. Interacts with STAT1. Interacts with CHUK/IKKA; this interaction induces PIAS1 phosphorylation. Interacts with PTK2/FAK1; the interaction promotes its sumoylation. Interacts with DDX5. Expressed in numerous tissues with highest level in testis. Belongs to the PIAS family.

Protein type: SUMO conjugating system; Transcription, coactivator/corepressor; Nuclear receptor co-regulator; EC 6.3.2.-

Chromosomal Location of Human Ortholog: 15q

Cellular Component: nucleoplasm; PML body; nuclear speck; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; DNA binding; androgen receptor binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription corepressor activity; ligase activity

Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; positive regulation of smooth muscle cell differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; cytokine and chemokine mediated signaling pathway; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; spermatogenesis; negative regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein-DNA complex assembly; regulation of cell proliferation