Rabbit PIASx Polyclonal Antibody | anti-PIASx antibody

PIASx (G24) Polyclonal Antibody

Cat. Num. AAA3001127

• Gene Names: PIAS2; DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: PIASx; Polyclonal Antibody; PIASx (G24) Polyclonal Antibody; E3 SUMO-protein ligase PIAS2; Protein inhibitor of activated STAT2; Protein inhibitor of activated STAT x; Msx-interacting zinc finger protein; Miz1; DAB2-interacting protein; DIP; Androgen receptor-interacting protein 3; ARIP3; PIAS-NY protein; PIAS; anti-PIASx antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PIAS2 (G24) polyclonal antibody detects endogenous levels of PIAS2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 621

• Applicable Applications for anti-PIASx antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 1-50 of Human PIASx.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PIASx (G24) pAb at 1:500 dilutionLane1:MCF-7 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:PC3 whole cell lysate (40ug) Lane4:The Testis tissue lysate of Mouse (40ug) Lane5:The Testis tissue lysate of Rat (40ug))

Related Product Information for anti-PIASx antibody

The IL-6-type family of cytokines, which includes IL-6 as well as a number of similar cytokines and growth factors, plays a significant role in regulating gene activation, proliferation and differentiation. Transcription factors of the Stat family are known to be involved in this signal transduction pathway, undergoing phosphorylation, dimerization and translocation to the nucleus upon activation. PIAS 1, for protein inhibitor of activated Stat1 (also designated Gu/RNA helicase II binding protein), binds specifically to Stat1, blocking Stat1 DNA-binding activity and inhibiting Stat1-mediated gene activation. PIAS 1 also binds to the Gu/RNA helicase II enzyme, leading to the proteolytic cleavage of Gu/RH-II. PIAS 3 similarly binds specifically to Stat3, blocking Stat3 DNA-binding activity and inhibiting Stat3-mediated gene activation.

NCBI and Uniprot Product Information

• NCBI GI #: 56699458
• NCBI GeneID: 9063
• NCBI Accession #: NP_004662.2
• NCBI GenBank Nucleotide #: NP_004662.2
• UniProt Accession #: O75928
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: E3 SUMO-protein ligase PIAS2 isoform beta
• NCBI Official Synonym Full Names: protein inhibitor of activated STAT 2
• NCBI Official Symbol: PIAS2
• NCBI Official Synonym Symbols: DIP; MIZ1; SIZ2; ARIP3; PIASX; ZMIZ4
• NCBI Protein Information: E3 SUMO-protein ligase PIAS2
• UniProt Protein Name: E3 SUMO-protein ligase PIAS2
• UniProt Gene Name: PIAS2
• UniProt Synonym Gene Names: PIASX; ARIP3; DIP; Miz1
• UniProt Entry Name: PIAS2_HUMAN

NCBI Description

This gene encodes a member of the protein inhibitor of activated STAT family, which function as SUMO E3 ligases and play important roles in many cellular processes by mediating the sumoylation of target proteins. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Isoforms of the encoded protein enhance the sumoylation of specific target proteins including the p53 tumor suppressor protein, c-Jun, and the androgen receptor. A pseudogene of this gene is located on the short arm of chromosome 4. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. [provided by RefSeq, Aug 2017]

Uniprot Description

PIAS2: Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. Binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2- alpha. Belongs to the PIAS family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 6.3.2.-; Nuclear receptor co-regulator; SUMO conjugating system; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: PML body; nuclear speck; nucleus

Molecular Function: protein binding; androgen receptor binding; DNA binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; transcription factor binding; ligase activity

Biological Process: protein sumoylation; transcription, DNA-dependent; regulation of osteoblast differentiation; negative regulation of transcription factor activity; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter