Rabbit Cardiotrophin-1 Polyclonal Antibody | anti-H31 antibody

Cardiotrophin-1 (H31) Polyclonal Antibody

Cat. Num. AAA3004118

• Gene Names: CTF1; CT1; CT-1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: Cardiotrophin-1; Polyclonal Antibody; Cardiotrophin-1 (H31) Polyclonal Antibody; CT-1; CTF1; Cardiotrophin1; CT1; Cardiotrophin 1; CT 1; anti-H31 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Cardiotrophin-1 (H31) polyclonal antibody detects endogenous levels of Cardiotrophin-1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 201

• Applicable Applications for anti-H31 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1-53 of Human CT-1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of Cardiotrophin-1 (H31) pAb at 1:500 dilutionLane1:PC3 whole cell lysate (40ug) Lane2:K562 whole cell lysate (20ug) Lane3:Jurkat whole cell lysate (40ug) Lane4:The Muscle tissue lysate of Mouse (40ug) Lane5:H9C2 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of Cardiotrophin-1 (H31) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.)

Related Product Information for anti-H31 antibody

Cardiotrophin-1 (CT-1) is a member of the IL-6 family of cytokines, which signal through gp130 receptor complexes. gp130 complexes with several different receptor subunits to transmit signals from Cardiotrophin-1, IL-6, LIF, OSM, CNTF and IL-11. Cardiotrophin-1 binds to and activates the leukemia inhibitory factor (LIF) receptor/gp130 receptor complex and has been shown to induce hypertrophy in cardiac myocytes in vitro. Cardiotrophin-1, a secreted protein expressed at high levels in myocardium during cardiogenesis, has been shown to promote proliferation and survival of embryonic cardiomyocytes, suggesting a role for Cardiotrophin-1 in the activation of gp130 during cardiac development.

NCBI and Uniprot Product Information

• NCBI GI #: 4503121
• NCBI GeneID: 1489
• NCBI Accession #: NP_001321.1
• NCBI GenBank Nucleotide #: NP_001321.1
• UniProt Accession #: Q16619
• Molecular Weight: ~ 21kDa
• NCBI Official Full Name: cardiotrophin-1 isoform 1
• NCBI Official Synonym Full Names: cardiotrophin 1
• NCBI Official Symbol: CTF1
• NCBI Official Synonym Symbols: CT1; CT-1
• NCBI Protein Information: cardiotrophin-1
• UniProt Protein Name: Cardiotrophin-1
• Protein Family: Cardiotrophin
• UniProt Gene Name: CTF1
• UniProt Synonym Gene Names: CT-1
• UniProt Entry Name: CTF1_HUMAN

NCBI Description

The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

CTF1: Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: leukemia inhibitory factor receptor binding; cytokine activity

Biological Process: nervous system development; cell proliferation; muscle development; cell-cell signaling; leukemia inhibitory factor signaling pathway; positive regulation of cell proliferation; neuron development