Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PI3KCB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB)
Rabbit PI3K beta Polyclonal Antibody | anti-PI3K beta antibody
PI3K beta antibody
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
PI3K beta antibody was purified by affinity chromatography
Synonyms
PI3K beta; Polyclonal Antibody; PI3K beta antibody; Polyclonal PI3K beta; anti-PI3K beta; PIK beta-3; PI3-kinase p110 subunit beta; PIK beta 3; Phosphatidylinositol-4; PIK3CB; 5-bisphosphate 3-kinase catalytic subunit beta form; anti-PI3K beta antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PI3K beta antibody was purified by affinity chromatography
Form/Format
Supplied in PBS buffer with 0.09% (W/V) sodium azide
Concentration
250 ug/ml (varies by lot)
Applicable Applications for anti-PI3K beta antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
ELISA: 0.25 ug/ml
IHC: 5-25 ug/ml
WB: 0.5-2.5 ug/ml
IHC: 5-25 ug/ml
WB: 0.5-2.5 ug/ml
Biological Significance
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine or serine/threonine kinase catalytic domains.
Cross-Reactivity
Human
Immunogen
PI3K beta antibody was raised in Rabbit using synthetic peptide selected from the C-terminal region of human PI3Kbeta as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PI3KCB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with PI3KCB antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB)
Western Blot (WB)
(Western blot analysis of antibodyin Jurkat cell lysate. PI3KCB (arrow) was detected using purified pAb. Secondary HRP-antirabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of antibodyin Jurkat cell lysate. PI3KCB (arrow) was detected using purified pAb. Secondary HRP-antirabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PI3K beta antibody
Rabbit polyclonal PI3K beta antibody
Product Categories/Family for anti-PI3K beta antibody
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Product Notes
The PI3K beta (Catalog #AAA5301715) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PI3K beta can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). ELISA: 0.25 ug/ml IHC: 5-25 ug/ml WB: 0.5-2.5 ug/ml. Researchers should empirically determine the suitability of the PI3K beta for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3K beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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