Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 12 % SDS PAGE gel and beta A4 Crystallin antibody at a dilution of 1:1000)
Rabbit beta A4 Crystallin Polyclonal Antibody
beta A4 Crystallin antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
beta A4 Crystallin antibody was purified by antigen-affinity chromatography
Synonyms
beta A4 Crystallin; Polyclonal Antibody; beta A4 Crystallin antibody; Polyclonal beta A4 Crystallin; Anti-beta A4 Crystallin; beta A Crystallin 4; CRYBA4; crystallin beta polypeptide A4; crystallin beta A4; beta-A4 crystallin; eye lens structural protein; beta-crystallin A4; beta A Crystallin-4; MCOPCT4; anti-beta A4 Crystallin antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
beta A4 Crystallin antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-beta A4 Crystallin antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
WB: 1:500-1:3000
Biological Significance
Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Beta-crystallins, the most heterogeneous, differ by the presence of the C-terminal extension.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 12 % SDS PAGE gel and beta A4 Crystallin antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 50 ug of whole cell lysate (A: mouse brain) using a 12 % SDS PAGE gel and beta A4 Crystallin antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: HeLa) using a 12 % SDS PAGE gel and beta A4 Crystallin antibody at a dilution of 1:1000)
Western Blot (WB)
(Western blot analysis of 30 ug of whole cell lysate (A: H1299; B: HeLa) using a 12 % SDS PAGE gel and beta A4 Crystallin antibody at a dilution of 1:1000)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4 antibody at 1:200 dilution.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Beta-crystallin A4 antibody at 1:200 dilution.)
Related Product Information for anti-beta A4 Crystallin antibody
Affinity purified Rabbit polyclonal beta A4 Crystallin antibody
Product Categories/Family for anti-beta A4 Crystallin antibody
Similar Products
Product Notes
The beta A4 Crystallin (Catalog #AAA837660) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's beta A4 Crystallin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the beta A4 Crystallin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta A4 Crystallin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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