Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Exosome complex exonuclease RRP44 (DIS3) Standard Curve.)
Mouse Exosome Complex Exonuclease RRP44 (DIS3) ELISA Kit | DIS3 elisa kit
Mouse Exosome Complex Exonuclease RRP44 (DIS3) ELISA Kit
Gene Names
Dis3; 2810028N01Rik
Reactivity
Mouse
Synonyms
Exosome Complex Exonuclease RRP44 (DIS3); Mouse Exosome Complex Exonuclease RRP44 (DIS3) ELISA Kit; DIS3; DKFZp667L1817; EXOSC11; FLJ10484; KIAA1008; MGC33035; RP11-342J4.3; RRP44; bA555G22.1; dis3p; 2810028N01Rik; DIS3 mitotic control; exosome component 11; mitotic control protein dis3 homolog; DIS3 elisa kit
Reactivity
Mouse
Specificity
Mouse Exosome complex exonuclease RRP44 (DIS3) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse DIS3. No significant cross-reactivity or interference between Mouse DIS3 and analogues was observed.
Sequence Length
958
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Exosome complex exonuclease RRP44 (DIS3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Exosome complex exonuclease RRP44 (DIS3) Standard Curve.)
Related Product Information for DIS3 elisa kit
Description: This Mouse Exosome complex exonuclease RRP44 (DIS3) ELISA Kit employs a two-site sandwich ELISA to quantitate DIS3 in samples. An antibody specific for DIS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DIS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: By randomly sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1999) cloned DIS3, which they designated KIAA1008. The deduced 928-amino acid protein shares 44.5% identity with the mitotic control protein Dis3 of S. pombe. PCR-ELISA detected low to moderate expression in all tissues and brain regions tested, with highest expression in testis and moderate expression in kidney, ovary, corpus callosum, spinal cord, and fetal brain. By sequence analysis of chromosome 13q21-q22 to identify a breast cancer susceptibility gene, Rozenblum et al. (2002) identified DIS3. The deduced protein contains 958 amino acids. Northern blot analysis detected ubiquitous expression.
Background: By randomly sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1999) cloned DIS3, which they designated KIAA1008. The deduced 928-amino acid protein shares 44.5% identity with the mitotic control protein Dis3 of S. pombe. PCR-ELISA detected low to moderate expression in all tissues and brain regions tested, with highest expression in testis and moderate expression in kidney, ovary, corpus callosum, spinal cord, and fetal brain. By sequence analysis of chromosome 13q21-q22 to identify a breast cancer susceptibility gene, Rozenblum et al. (2002) identified DIS3. The deduced protein contains 958 amino acids. Northern blot analysis detected ubiquitous expression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
exosome complex exonuclease RRP44 isoform 1
NCBI Official Synonym Full Names
DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease
NCBI Official Symbol
Dis3
NCBI Official Synonym Symbols
2810028N01Rik
NCBI Protein Information
exosome complex exonuclease RRP44
UniProt Protein Name
Exosome complex exonuclease RRP44
Protein Family
UniProt Gene Name
Dis3
UniProt Synonym Gene Names
Kiaa1008; Rrp44
UniProt Entry Name
RRP44_MOUSE
Research Articles on DIS3
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Product Notes
The Mouse DIS3 dis3 (Catalog #AAA9717882) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717882 ELISA Kit recognizes Mouse DIS3. It is sometimes possible for the material contained within the vial of "Exosome Complex Exonuclease RRP44 (DIS3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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