Immunofluorescence
(Immunofluorescent staining using PI3 kinase p110 beta antibodyImmunofluorescence analysis of methanol-fixed HeLa, using PI3 kinase p110 beta antibody at 1:200 dilution.)
Rabbit PI3 Polyclonal Antibody | anti-PI3 antibody
PI3 kinase p110 beta antibody
Applications
Immunofluorescence, Immunocytochemistry, Western Blot
Purity
PI3 kinase p110 beta antibody was purified by antigen-affinity chromatography
Synonyms
PI3; Polyclonal Antibody; PI3 kinase p110 beta antibody; Polyclonal PI3 kinase p110 beta; Anti-PI3 kinase p110 beta; PI3K; PI3-kinase p110 subunit beta; DKFZp779K1237; P110BETA; MGC133043; PI3 kinase p110 beta; PIK3CB; PI3K-beta; ptdIns-3-kinase subunit beta; PI kinase p110 beta-3; PIK3C1; PtdIns-3-kinase p110; PI3KBETA; PI kinase p110 beta 3; anti-PI3 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PI3 kinase p110 beta antibody was purified by antigen-affinity chromatography
Form/Format
Supplied as a concentrated soloution containing 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
117
Applicable Applications for anti-PI3 antibody
Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
IF:1:100-1:500
ICC:1:100-1:500
WB: 1:500-1:3000
ICC:1:100-1:500
WB: 1:500-1:3000
Biological Significance
Phosphoinositide 3-kinases phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. They have been implicated as participants in signaling pathways regulating cell growth by virtue of their activation in response to various mitogenic stimuli. PI3Ks are composed of a 110-kD catalytic subunit, such as PIK3CB, and an 85-kD adaptor subunit.
Cross-Reactivity
Human, Mouse
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Immunofluorescence
(Immunofluorescent staining using PI3 kinase p110 beta antibodyImmunofluorescence analysis of methanol-fixed HeLa, using PI3 kinase p110 beta antibody at 1:200 dilution.)
Immunofluorescence
(Immunofluorescent staining using PI3 kinase p110 beta antibodyImmunofluorescence analysis of methanol-fixed HeLa, using PI3 kinase p110 beta antibody at 1:200 dilution.)
Western Blot
(Western blot analysis using PI3 kinase p110 beta antibodyWestern blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and PI3 kinase p110 beta antibody at a dilution of 1:1000)
Western Blot
(Western blot analysis using PI3 kinase p110 beta antibodyWestern blot analysis of 30 ug of whole cell lysate (A: H1299) using a 7.5 % SDS PAGE gel and PI3 kinase p110 beta antibody at a dilution of 1:1000)
Western Blot
(Western blot analysis using PI3 kinase p110 beta antibodyWestern blot analysis of 50 ug of whole cell lysate (A: C2C12; B: C2C12+AEA10mM) using a 7.5 % SDS PAGE gel and PI3 kinase p110 beta antibody at a dilution of 1:1000)
Western Blot
(Western blot analysis using PI3 kinase p110 beta antibodyWestern blot analysis of 50 ug of whole cell lysate (A: C2C12; B: C2C12+AEA10mM) using a 7.5 % SDS PAGE gel and PI3 kinase p110 beta antibody at a dilution of 1:1000)
Related Product Information for anti-PI3 antibody
Affinity purified Rabbit polyclonal PI3 kinase p110 beta antibody
Product Categories/Family for anti-PI3 antibody
NCBI and Uniprot Product Information
Uniprot Description
elafin: Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Protein type: Secreted; Extracellular matrix; Secreted, signal peptide; Inhibitor
Chromosomal Location of Human Ortholog: 20q13.12
Cellular Component: proteinaceous extracellular matrix
Molecular Function: serine-type endopeptidase inhibitor activity; endopeptidase inhibitor activity
Biological Process: copulation
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Product Notes
The PI3 pi3 (Catalog #AAA837209) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's PI3 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunocytochemistry (ICC), Western Blot (WB). IF:1:100-1:500 ICC:1:100-1:500 WB: 1:500-1:3000. Researchers should empirically determine the suitability of the PI3 pi3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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