Western Blot (WB)
(Sample Data: Western blot using affinity purified Anti-PCNA antibody (1:1000) shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Load: ~25 ug of lysate per lane.)
Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody
Anti-PCNA
Reactivity
Human, Monkey, Dog, Mouse, Rat, Bovine, Chicken, Fish, Xenopus
Applications
ELISA, Western Blot
Purity
Immunoaffinity chromatography
Synonyms
PCNA; Polyclonal Antibody; Anti-PCNA; anti-PCNA antibody
Host
Rabbit
Reactivity
Human, Monkey, Dog, Mouse, Rat, Bovine, Chicken, Fish, Xenopus
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the human PCNA protein
Purity/Purification
Immunoaffinity chromatography
Form/Format
0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH7.2 + 0.01% (w/v) Sodium Azide
Applicable Applications for anti-PCNA antibody
ELISA (EIA), Western Blot (WB)
Immunogen
The antibody was produced against synthesized peptide corresponding to an internal region of human PCNA
Preparation and Storage
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot using affinity purified Anti-PCNA antibody (1:1000) shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Load: ~25 ug of lysate per lane.)
Western Blot (WB)
(Sample Data: Western blot using affinity purified Anti-PCNA antibody (1:1000) shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Load: ~25 ug of lysate per lane.)
Related Product Information for anti-PCNA antibody
PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro (1). PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H (2).
Product Categories/Family for anti-PCNA antibody
References
1. Hasan, S. et al: Transcription coactivator p300 binds PCNA and may have a role in DNA repair synthesis. Nature 410: 387-391, 2001.
2. Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
2. Poot, R. A.et al: The Williams syndrome transcription factor interacts with PCNA to target chromatin remodelling by ISWI to replication foci. Nature Cell Biol. 6: 1236-1244, 2004.
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Product Notes
The PCNA (Catalog #AAA516130) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PCNA reacts with Human, Monkey, Dog, Mouse, Rat, Bovine, Chicken, Fish, Xenopus and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the PCNA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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