Western Blot (WB)
(Western blot analysis of extracts from various cell lines:Lane1-8: Hela, 293T, Cos-7, NIH/3T3, CHO-K1, MDCK, PC12, C6Primary antibody: 1: 20000)
Rabbit PCNA Polyclonal Antibody | anti-PCNA antibody
PCNA Rabbit pAb
Gene Names
PCNA; ATLD2
Reactivity
Human, Monkey, Mouse, Chinese Hamster, Dog, Rat
Applications
Western Blot
Synonyms
PCNA; Polyclonal Antibody; PCNA Rabbit pAb; anti-PCNA antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Chinese Hamster, Dog, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
PCNA antibody detects endogenous levels of total PCNA protein.
Sequence Length
261
Applicable Applications for anti-PCNA antibody
Western Blot (WB)
Application Notes
WB: 1: 5000-20000
Source Note
PCNA polyclonal antibody is produced by immunizing rabbit with a synthetic peptide corresponding to the internal residues of human PCNA.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Western Blot (WB)
(Western blot analysis of extracts from various cell lines:Lane1-8: Hela, 293T, Cos-7, NIH/3T3, CHO-K1, MDCK, PC12, C6Primary antibody: 1: 20000)
Western Blot (WB)
(Western blot analysis of extracts from various cell lines:Lane1-8: Hela, 293T, Cos-7, NIH/3T3, CHO-K1, MDCK, PC12, C6Primary antibody: 1: 20000)
Related Product Information for anti-PCNA antibody
PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
Product Categories/Family for anti-PCNA antibody
References
1. Kelman, Z. and O'Donnell, M. (1995) Nucleic Acids Res. 23, 3613-3620.2. Krishna, T. S. et al. (1994) Cell 79, 1233-1243.3. Maga, G. and Hubscher, U. (2003) J. Cell Sci. 116, 3051-3060.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted MW: 29kDa
NCBI Official Full Name
PCNA, partial
NCBI Official Synonym Full Names
proliferating cell nuclear antigen
NCBI Official Symbol
PCNA
NCBI Official Synonym Symbols
ATLD2
NCBI Protein Information
proliferating cell nuclear antigen
UniProt Protein Name
Proliferating cell nuclear antigen
Protein Family
UniProt Gene Name
PCNA
NCBI Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Uniprot Description
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
Research Articles on PCNA
Similar Products
Product Notes
The PCNA pcna (Catalog #AAA435018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PCNA Rabbit pAb reacts with Human, Monkey, Mouse, Chinese Hamster, Dog, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1: 5000-20000. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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