Immunohistochemistry (IHC)
(Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit PCNA / Cyclin Polyclonal Antibody | anti-PCNA antibody
Rabbit Polyclonal to Human PCNA / Cyclin
Usage: Immunohistochemistry: Was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 2.5 ug/ml.
Immunohistochemistry (IHC)
(Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-PCNA antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB)
(Anti-PCNA Antibody - Western Blot. Western blot of affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 ((800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.)
Western Blot (WB)
(Anti-PCNA Antibody - Western Blot. Western blot of affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10000 dilution of IRDye 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 ((800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Uniprot Description
PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.
Protein type: Cell cycle regulation; DNA replication
Chromosomal Location of Human Ortholog: 20pter-p12
Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; nuclear replication fork; DNA replication factor C complex; nucleus
Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; MutLalpha complex binding; purine-specific mismatch base pair DNA N-glycosylase activity; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding
Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; leading strand elongation; response to lipid; DNA repair; telomere maintenance via semi-conservative replication; G1/S-specific transcription in mitotic cell cycle; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; regulation of DNA replication; G1/S transition of mitotic cell cycle
Disease: Ataxia-telangiectasia-like Disorder 2
Research Articles on PCNA
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Product Notes
The PCNA pcna (Catalog #AAA240535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human PCNA / Cyclin reacts with Bovine, Chicken, Dog, Fish, Xenopus, Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PCNA / Cyclin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA (1:2000 - 1:8000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000) Usage: Immunohistochemistry: Was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for was determined to be 2.5 ug/ml. Researchers should empirically determine the suitability of the PCNA pcna for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCNA / Cyclin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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