Peptidyl Arginine Deimina Type III Recombinant Protein | PADI3 recombinant protein

Recombinant Peptidyl Arginine Deimina Type III (PADI3)

Cat. Num. AAA2030501

• Gene Names: PADI3; PAD3; PDI3
• Reactivity: Human
• Applications: SDS-Page, Western Blot
• Purity: >98%
• Synonyms: Peptidyl Arginine Deimina Type III; Recombinant Peptidyl Arginine Deimina Type III (PADI3); PADI3 recombinant protein

• Host: E. coli
• Reactivity: Human
• Purity/Purification: >98%
• Form/Format: 100mM NaHCO₃, 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Concentration: Original Concentration: 200µg/mL (varies by lot)

• Applicable Applications for PADI3 recombinant protein: Positive Control, Immunogen, SDS-PAGE, WB; ; (May be suitable for use in other assays to be determined by the end user.)
• Source: Prokaryotic expression
• Residues: Met1~Pro300
• Tags: N-terminal His-Tag
• Tissue Specificity: Brain, Pancreas, Spleen
• Subcellular Location: Cytoplasm
• Traits: Freeze-dried powder
• Predicted isoelectric point: 5.0
• Predicted Molecular Mass: 37.5kDa
• Accurate Molecular Mass: 35kDa as determined by SDS-PAGE reducing conditions
• Usage: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:Avoid repeated freeze/thaw cycles.; Store at 2-8°C for one month.; Aliquot and store at -80°C for 12 months.; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 122939161
• NCBI GeneID: 51702
• NCBI Accession #: NP_057317.2
• NCBI GenBank Nucleotide #: NM_016233.2
• NCBI Official Full Name: protein-arginine deiminase type-3
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type III
• NCBI Official Symbol: PADI3
• NCBI Official Synonym Symbols: PAD3; PDI3
• NCBI Protein Information: protein-arginine deiminase type-3
• UniProt Protein Name: Protein-arginine deiminase type-3
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: PADI3
• UniProt Synonym Gene Names: PAD3; PDI3
• UniProt Entry Name: PADI3_HUMAN

NCBI Description

This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type III enzyme modulates hair structural proteins, such as filaggrin in the hair follicle and trichohyalin in the inner root sheath, during hair follicle formation. Together with the type I enzyme, this enzyme may also play a role in terminal differentiation of the epidermis. This gene exists in a cluster with four other paralogous genes. [provided by RefSeq, Jul 2008]

Uniprot Description

PADI3: Catalyzes the deimination of arginine residues of proteins. Belongs to the protein arginine deiminase family.

Protein type: Hydrolase; EC 3.5.3.15

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; protein-arginine deiminase activity

Biological Process: peptidyl-citrulline biosynthetic process from peptidyl-arginine