Rabbit p21CIP1 Polyclonal Antibody | anti-p21CIP1 antibody

Anti-p21CIP1

Cat. Num. AAA515896

• Reactivity: Western blot human p21CIP1; p21CIP1 from other species may also be detectable.
• Applications: Western Blot
• Purity: Affinity Chromatography
• Synonyms: p21CIP1; Polyclonal Antibody; Anti-p21CIP1; anti-p21CIP1 antibody

• Host: Rabbit
• Reactivity: Western blot human p21CIP1; p21CIP1 from other species may also be detectable.
• Clonality: Polyclonal
• Specificity: Recognizes the p21CIP1 (CDKN1) protein
• Purity/Purification: Affinity Chromatography
• Form/Format: PBS, pH7.4, 0.02%NaN3, 50% glycerol

• Applicable Applications for anti-p21CIP1 antibody: Western Blot (WB)
• Application Notes: WB: 19396:20395
• Immunogen: Synthetic peptide corresponding to amino acids 143-147 of human p21CIP1
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Representative Western blot with Anti-p21CIP1 (1:1000) using 20ng of GST-tagged human recombinant p21CIP1.)

Western Blot (WB)

(Sample Data: Representative Western blot with Anti-p21CIP1 (1:1000) using 3 ug, 10 ug and 30 ug of THP1cell lysate.)

Related Product Information for anti-p21CIP1 antibody

CIP1 (Cyclin-Dependent Kinase Inhibitor 1A) regulates cell cycle progression, terminal differentiation, and apoptosis (1). CIP1 was shown to be induced by p53 and to be a potent inhibitor of cyclin-dependent kinase (CDK) activity. DNA damage leads to increased expression of CIP1 in cyclin E-containing complexes and to an associated decrease in cyclin-dependent kinase activity. CIP1 is a critical downstream effector in the p53-specific pathway of growth control in mammalian cells (2).

Product Categories/Family for anti-p21CIP1 antibody

Primary Antibodies; Cancer; Cell Cycle

References

1. el-Deiry, W S. et al: WAF1/CIP1 is induced in p53-mediated G1 arrest and apoptosis. Cancer Res. 1994;54(5):1169-74
2. Wagner, M. et al: Cyclin-dependent kinase-inhibitor 1 (CDKN1A) in the squamous epithelium of the oropharynx: possible implications of molecular biology and compartmentation. Anticancer Res. 2001; 21(1A):333-45.