Western Blot (WB)
(Sample Data: Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.)
Mouse CHK2 Monoclonal Antibody | anti-CHK2 antibody
Anti-CHK2
Reactivity
Western blot CHK2 from human, mouse cellsCHK2 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
CHK2; Monoclonal Antibody; Anti-CHK2; anti-CHK2 antibody
Host
Mouse
Reactivity
Western blot CHK2 from human, mouse cells
CHK2 from other species may also be detectable.
CHK2 from other species may also be detectable.
Clonality
Monoclonal
Isotype
Mouse, IgG1
Specificity
Recognizes the CHK2 protein
Purity/Purification
Affinity Chromatography
Form/Format
0.1M TRIS pH8.0, 0.1M glycine
Applicable Applications for anti-CHK2 antibody
Western Blot (WB)
Application Notes
WB: 22110:23109
Immunogen
Human full length recombinant CHK1
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.)
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) with Anti-CHK2 using 20 ng of GST-tagged human full length recombinant CHK1 and CHK2.)
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) with Anti-CHK2 using 3 ug, 10 ug, 30ug of 293T cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot (1:1000) with Anti-CHK2 using 3 ug, 10 ug, 30ug of 293T cell lysate.)
Related Product Information for anti-CHK2 antibody
CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type Chk2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative Chk2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).
Product Categories/Family for anti-CHK2 antibody
References
1. Matsuoka, S. et al: Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998 Dec 4;282(5395):1893-7.
2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.
2. Chehab NH. et al: Chk2/hCds1 functions as a DNA damage checkpoint in G(1) by stabilizing p53. Genes Dev. 2000 Feb 1;14(3):278-88.
Similar Products
Product Notes
The CHK2 (Catalog #AAA515908) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CHK2 reacts with Western blot CHK2 from human, mouse cells CHK2 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's CHK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 22110:23109. Researchers should empirically determine the suitability of the CHK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CHK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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