Rabbit anti-Human OR2AP1 Polyclonal Antibody | anti-OR2AP1 antibody

OR2AP1 Antibody

Cat. Num. AAA9508130

• Gene Names: OR2AP1; OR2AP1P
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: OR2AP1; Polyclonal Antibody; OR2AP1 Antibody; anti-OR2AP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The OmniKine™ Human BD-3 ELISA is capable of recognizing both recombinant and naturally produced Human BD-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 309

• Applicable Applications for anti-OR2AP1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:1000
• Assay Type: Quantitative Sandwich
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from Jurkat cells, using OR2AP1 Antibody. The lane on the right is blocked with the synthesized peptide.)

Related Product Information for anti-OR2AP1 antibody

Principle of the Assay: The OmniKine™ Human BD-3 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BD-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BD-3 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human BD-3, also known as Beta-Defensin 3, is a 67 amino acid cytokine protein encoded by the DEFB103A gene located at locus 8p23.1 on chromosome 8. After signal sequence processing and maturation, the cytokine becomes 45 amino acid residues long (from 23 to 67). Human BD-3 is known to exhibit antimicrobial activity against Gram-positive bacteria, S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Moreover, the cytokine kills multiresistant S.aureus and vancomycin-resistant E.faecium. This particular BD protein is expressed highly in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It has much lower expression in salivary glands, bone marrow, colon, stomach, polyps and larynx along with no expression in the small intestine. Furthermore, induction of BD-3 is achieved through bacterial infection or detection of lipopolysaccharides (LPS) and by IFN-gamma.

NCBI and Uniprot Product Information

• NCBI GI #: 385137115
• NCBI GeneID: 121129
• NCBI Accession #: NP_001245214.1
• NCBI GenBank Nucleotide #: NM_001258285.1
• UniProt Accession #: Q8NGE2
• Molecular Weight: 34 kDa
• NCBI Official Full Name: olfactory receptor 2AP1
• NCBI Official Synonym Full Names: olfactory receptor family 2 subfamily AP member 1
• NCBI Official Symbol: OR2AP1
• NCBI Official Synonym Symbols: OR2AP1P
• NCBI Protein Information: olfactory receptor 2AP1
• UniProt Protein Name: Olfactory receptor 2AP1
• Protein Family: Olfactory receptor
• UniProt Gene Name: OR2AP1
• UniProt Synonym Gene Names: OR2AP1P

NCBI Description

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Uniprot Description

Odorant receptor.