Human RAGE ELISA Kit | RAGE elisa kit

RAGE (Human) OmniKine ELISA Kit

Cat. Num. AAA9502071

• Gene Names: AGER; RAGE; SCARJ1
• Reactivity: Human
• Synonyms: RAGE; RAGE (Human) OmniKine ELISA Kit; Advanced glycosylation end product-specific receptor; Receptor for advanced glycosylation end products; RAGE elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human RAGE ELISA is capable of recognizing both recombinant and naturally produced Human RAGE proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 404

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human RAGE ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human RAGE proteins within the range of 63-4000 pg/ml.)

Related Product Information for RAGE elisa kit

Principle of the Assay: The OmniKine™ Human RAGE ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human RAGE concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human RAGE while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: RAGE is able to phosphorylate several exogenous substrates and undergo auto-phosphorylation. Phosphorylation appears to increase enzymatic activity. RAGE is expressed in the heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. RAGE is also detected in retinas.

NCBI and Uniprot Product Information

• NCBI GI #: 10835203
• NCBI GeneID: 177
• NCBI Accession #: NP_001127.1
• NCBI GenBank Nucleotide #: NM_001136.4
• UniProt Accession #: Q15109; Q15279; Q3L1R4; Q3L1R5; Q3L1R6; Q3L1R7; Q3L1R8; Q3L1S0; A2BFI7; A6NKF0; A7Y2U9; B0V176
• Molecular Weight: 41,098 Da
• NCBI Official Full Name: advanced glycosylation end product-specific receptor isoform 1
• NCBI Official Synonym Full Names: advanced glycosylation end-product specific receptor
• NCBI Official Symbol: AGER
• NCBI Official Synonym Symbols: RAGE; SCARJ1
• NCBI Protein Information: advanced glycosylation end product-specific receptor
• UniProt Protein Name: Advanced glycosylation end product-specific receptor
• UniProt Gene Name: AGER
• UniProt Synonym Gene Names: RAGE

NCBI Description

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847). [provided by RefSeq, May 2011]

Uniprot Description

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Can also bind oligonucleotides.