Human Gas6 ELISA Kit | GAS6 elisa kit

Gas6 (Human) OmniKine ELISA Kit

Cat. Num. AAA9502053

• Gene Names: GAS6; AXSF; AXLLG
• Reactivity: Human
• Synonyms: Gas6; Gas6 (Human) OmniKine ELISA Kit; Growth arrest-specific protein 6; AXL receptor tyrosine kinase ligand; GAS6 elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human Gas6 ELISA is capable of recognizing both recombinant and naturally produced Human Gas6 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 678

• Samples: Cell lysates, serum and plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: 125-8000 pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human Gas6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Gas6 proteins within the range of 125-8000 pg/ml.)

Related Product Information for GAS6 elisa kit

Principle of the Assay: The OmniKine™ Human Gas6 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human Gas6 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human Gas6 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.!!Background/Introduction: Gas6 is a ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER, whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. Gas6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen and is proteolytically processed after secretion to yield an N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

NCBI and Uniprot Product Information

• NCBI GI #: 4557617
• NCBI GeneID: 2621
• NCBI Accession #: NP_000811.1
• NCBI GenBank Nucleotide #: NM_000820.3
• UniProt Accession #: Q14393; Q6IMN1; Q7Z7N3; B3KRQ7; B3KVL4; E9PBL7
• Molecular Weight: 41,986 Da
• NCBI Official Full Name: growth arrest-specific protein 6
• NCBI Official Synonym Full Names: growth arrest specific 6
• NCBI Official Symbol: GAS6
• NCBI Official Synonym Symbols: AXSF; AXLLG
• NCBI Protein Information: growth arrest-specific protein 6
• UniProt Protein Name: Growth arrest-specific protein 6
• Protein Family: Growth arrest-specific protein
• UniProt Gene Name: GAS6
• UniProt Synonym Gene Names: AXLLG; GAS-6

NCBI Description

This gene encodes a gamma-carboxyglutamic acid (Gla)-containing protein thought to be involved in the stimulation of cell proliferation. This gene is frequently overexpressed in many cancers and has been implicated as an adverse prognostic marker. Elevated protein levels are additionally associated with a variety of disease states, including venous thromboembolic disease, systemic lupus erythematosus, chronic renal failure, and preeclampsia. [provided by RefSeq, Aug 2014]

Uniprot Description

GAS6: Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ligand, receptor tyrosine kinase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: cytoplasm; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi lumen

Molecular Function: calcium ion binding; caspase inhibitor activity; molecular adaptor activity; phosphatidylserine binding; protein binding; protein tyrosine kinase activator activity; receptor binding; receptor ligand activity; receptor tyrosine kinase binding; voltage-gated calcium channel activity

Biological Process: activation of protein kinase B activity; apoptosis; apoptotic cell clearance; cell adhesion; cell cycle arrest; cell migration; cell proliferation; cell-substrate adhesion; cellular protein metabolic process; cellular response to starvation; entry of virus into host cell; enzyme linked receptor protein signaling pathway; ER to Golgi vesicle-mediated transport; fusion of virus membrane with host plasma membrane; leukocyte migration; negative regulation of apoptosis; negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; negative regulation of interferon-gamma production; negative regulation of interleukin-1 secretion; negative regulation of interleukin-6 production; negative regulation of protein import into nucleus, translocation; negative regulation of transcription factor activity; negative regulation of transcription, DNA-dependent; negative regulation of tumor necrosis factor production; neuron migration; organ regeneration; peptidyl-serine phosphorylation; phagocytosis; platelet activation; platelet degranulation; positive regulation of cytokine and chemokine mediated signaling pathway; positive regulation of fibroblast proliferation; positive regulation of glomerular filtration; positive regulation of natural killer cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of phagocytosis; positive regulation of protein export from nucleus; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; positive regulation of TOR signaling pathway; post-translational protein modification; protein amino acid phosphorylation; protein kinase B signaling; receptor-mediated virion attachment to host cell; regulation of growth; signal peptide processing; signal transduction; viral genome replication