Typical Testing Data/Standard Curve (for reference only)
Human BD-3 CLIA Kit | BD-3 clia kit
BD-3 (Human) LumiAb ELISA Kit
Gene Names
DEFB103B; BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103
Reactivity
Human
Synonyms
BD-3; BD-3 (Human) LumiAb ELISA Kit; Human BD-3; Beta-defensin 103; Beta-defensin 3; DEFB-3; HBD3; BD3; DEFB103; DEFB3; BD-3 clia kit
Reactivity
Human
Specificity
The LumiAb™ Human BD-3 ELISA is capable of recognizing both recombinant and naturally produced Human BD-3 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
Sequence Length
67
Assay Type
Quantitative Sandwich
Preparation and Storage
Store at 4 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BD-3 clia kit
Principle of the Assay: The LumiAb™ Human BD-3 Chemiluminescent ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human BD-3 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BD-3 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a sensitive luminescent reaction to ensue upon substrate addition. When the Peroxide Enhancer solution is added, the reaction catalyzed by peroxidase yields light that is representative of the antigen concentration. After a brief incubation, the microplate can be read with a luminometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Background/Introduction: Human BD-3, also known as Beta-Defensin 3, is a 67 amino acid cytokine protein encoded by the DEFB103A gene located at locus 8p23.1 on chromosome 8. After signal sequence processing and maturation, the cytokine becomes 45 amino acid residues long (from 23 to 67). Human BD-3 is known to exhibit antimicrobial activity against Gram-positive bacteria, S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Moreover, the cytokine kills multiresistant S.aureus and vancomycin-resistant E.faecium. This particular BD protein is expressed highly in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It has much lower expression in salivary glands, bone marrow, colon, stomach, polyps and larynx along with no expression in the small intestine. Furthermore, induction of BD-3 is achieved through bacterial infection or detection of lipopolysaccharides (LPS) and by IFN-gamma.
Background/Introduction: Human BD-3, also known as Beta-Defensin 3, is a 67 amino acid cytokine protein encoded by the DEFB103A gene located at locus 8p23.1 on chromosome 8. After signal sequence processing and maturation, the cytokine becomes 45 amino acid residues long (from 23 to 67). Human BD-3 is known to exhibit antimicrobial activity against Gram-positive bacteria, S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Moreover, the cytokine kills multiresistant S.aureus and vancomycin-resistant E.faecium. This particular BD protein is expressed highly in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. It has much lower expression in salivary glands, bone marrow, colon, stomach, polyps and larynx along with no expression in the small intestine. Furthermore, induction of BD-3 is achieved through bacterial infection or detection of lipopolysaccharides (LPS) and by IFN-gamma.
Product Categories/Family for BD-3 clia kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
beta-defensin 103
NCBI Official Synonym Full Names
defensin beta 103B
NCBI Official Symbol
DEFB103B
NCBI Official Synonym Symbols
BD-3; HBD3; HBP3; DEFB3; HBD-3; HBP-3; DEFB-3; DEFB103
NCBI Protein Information
beta-defensin 103
UniProt Protein Name
Beta-defensin 103
UniProt Gene Name
DEFB103A
UniProt Synonym Gene Names
BD3; DEFB103; DEFB3; BD-3; DEFB-3; HBD3; hBD-3
UniProt Entry Name
D103A_HUMAN
NCBI Description
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 103, which has broad spectrum antimicrobial activity and may play an important role in innate epithelial defense. [provided by RefSeq, Oct 2014]
Uniprot Description
defensin, beta 3: Exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. Kills multiresistant S.aureus and vancomycin-resistant E.faecium. No significant hemolytic activity was observed. Belongs to the beta-defensin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 8p23.1
Cellular Component: Golgi lumen; extracellular region
Biological Process: defense response to bacterium; innate immune response; positive regulation of biosynthetic process of antibacterial peptides active against Gram-positive bacteria
Research Articles on BD-3
Similar Products
Product Notes
The Human BD-3 defb103a (Catalog #AAA9510758) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9510758 CLIA Kit recognizes Human BD-3. It is sometimes possible for the material contained within the vial of "BD-3, CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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