Human MBL ELISA Kit | MBL elisa kit

MBL (Human) OmniKine ELISA Kit

Cat. Num. AAA9502061

• Gene Names: MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• Reactivity: Human
• Synonyms: MBL; MBL (Human) OmniKine ELISA Kit; Mannose-binding protein C; Collectin-1; MBP1; Mannan-binding protein; Mannose-binding lectin; MBL elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human MBL ELISA is capable of recognizing both recombinant and naturally produced Human MBL proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 248

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MBL elisa kit

Principle of the Assay: The OmniKine™ Human MBL ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human MBL concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human MBL while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: MBL is a calcium-dependent lectin involved in innate immune defense. It binds mannose, fructose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. MPL also binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. MBL may bind DNA. MBL consists of an oligomeric complex of 3 or more homotrimers. This lectin interacts with MASP1 and MASP2. Interactions with MEP1A and MEP1B may inhibit their catalytic activities. Genetic variations in MBL2 influence susceptibility to hepatitis B virus (HBV) infection. There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.

NCBI and Uniprot Product Information

• NCBI GI #: 4557739
• NCBI GeneID: 4153
• NCBI Accession #: NP_000233.1
• NCBI GenBank Nucleotide #: NM_000242.2
• UniProt Accession #: P11226; Q4VB12; Q4VB13; Q4VB14; Q5SQS3; Q86SI4; Q96KE4; Q96TF7; Q96TF8; Q96TF9
• Molecular Weight: 26,144 Da
• NCBI Official Full Name: mannose-binding protein C
• NCBI Official Synonym Full Names: mannose binding lectin 2
• NCBI Official Symbol: MBL2
• NCBI Official Synonym Symbols: MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC
• NCBI Protein Information: mannose-binding protein C
• UniProt Protein Name: Mannose-binding protein C
• Protein Family: MreB-like protein
• UniProt Gene Name: MBL2
• UniProt Synonym Gene Names: COLEC1; MBL; MBP-C

NCBI Description

This gene encodes the soluble mannose-binding lectin or mannose-binding protein found in serum. The protein encoded belongs to the collectin family and is an important element in the innate immune system. The protein recognizes mannose and N-acetylglucosamine on many microorganisms, and is capable of activating the classical complement pathway. Deficiencies of this gene have been associated with susceptibility to autoimmune and infectious diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

MBL2: Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: cell surface; collagen; extracellular region; extracellular space

Molecular Function: calcium ion binding; calcium-dependent protein binding; mannose binding; protein binding; receptor binding; serine-type endopeptidase activity

Biological Process: acute-phase response; complement activation; complement activation, classical pathway; complement activation, lectin pathway; defense response to bacterium; defense response to Gram-positive bacterium; innate immune response; killing by host of symbiont cells; negative regulation of viral process; opsonization; positive regulation of phagocytosis; proteolysis; response to oxidative stress

Disease: Mannose-binding Protein Deficiency