Human IL-28A ELISA Kit | IL-28A elisa kit

IL-28A (Human) OmniKine ELISA Kit

Cat. Num. AAA9501972

• Gene Names: IFNL2; IL28A; IL-28A
• Reactivity: Human
• Synonyms: IL-28A; IL-28A (Human) OmniKine ELISA Kit; Interleukin-28A; Cytokine Zcyto20; Interferon lambda-2; IL-28A elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human IL-28A ELISA is capable of recognizing both recombinant and naturally produced Human IL-28A proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 200

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human IL-28A ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human IL-28A proteins within the range of 125-8000 pg/ml.)

Related Product Information for IL-28A elisa kit

Principle of the Assay: The OmniKine™ Human IL-28A ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-28A concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-28A while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: The OmniKine™ Human IL-27 ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human IL-27 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human IL-27 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

NCBI and Uniprot Product Information

• NCBI GI #: 26024321
• NCBI GeneID: 282616
• NCBI Accession #: NP_742150.1
• NCBI GenBank Nucleotide #: NM_172138.1
• UniProt Accession #: Q8IZJ0; Q45KQ8; Q6VN55; Q8IWL7
• Molecular Weight: 22,288 Da
• NCBI Official Full Name: interferon lambda-2
• NCBI Official Synonym Full Names: interferon lambda 2
• NCBI Official Symbol: IFNL2
• NCBI Official Synonym Symbols: IL28A; IL-28A
• NCBI Protein Information: interferon lambda-2
• UniProt Protein Name: Interferon lambda-2
• UniProt Gene Name: IFNL2
• UniProt Synonym Gene Names: IL28A; ZCYTO20; IFN-lambda-2; IL-28A

NCBI Description

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

Uniprot Description

IL28A: Cytokine with immunomodulatory activity. Up-regulates MHC class I antigen expression. Displays potent antiviral activity. Also displays antitumor activity. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: extracellular space; intracellular

Molecular Function: cytokine activity; receptor binding

Biological Process: defense response to virus; innate immune response; JAK-STAT cascade; mucosal immune response; positive regulation of immune response; regulation of T cell activation