Human PSA ELISA Kit | PSA elisa kit

PSA (Human) OmniKine ELISA Kit

Cat. Num. AAA9502057

• Gene Names: KLK3; APS; PSA; hK3; KLK2A1
• Reactivity: Human
• Synonyms: PSA; PSA (Human) OmniKine ELISA Kit; Prostate-specific antigen; Gamma-seminoprotein; Seminin; P-30 antigen; Semenogelase; PSA elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human PSA ELISA is capable of recognizing both recombinant and naturally produced Human PSA proteins. The antigens listed below were tested at 600 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 238

• Assay Type: Quantitative Sandwich
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The OmniKine Human PSA ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human PSA proteins within the range of 938-60000 pg/ml.)

Related Product Information for PSA elisa kit

Principle of the Assay: The OmniKine™ Human PSA ELISA Kit contains the components necessary for quantitative determination of natural or recombinant Human PSA concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human PSA while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of nonspecific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Kallikrein 3/PSA (prostate specific antigen) hydrolyzes semenogelin- 1, leading to the liquefaction of the seminal coagulum. Activity is strongly inhibited by Zn2+. PSA is 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders.

NCBI and Uniprot Product Information

• NCBI GI #: 71834853
• NCBI GeneID: 354
• NCBI Accession #: NP_001025218.1
• NCBI GenBank Nucleotide #: NM_001030047.1
• UniProt Accession #: P07288; Q15096; Q16272; Q86TG8; Q8IXI4; C9JXH3; G3V0H4; G3XAE3
• Molecular Weight: 25,116 Da
• NCBI Official Full Name: prostate-specific antigen isoform 3 preproprotein
• NCBI Official Synonym Full Names: kallikrein related peptidase 3
• NCBI Official Symbol: KLK3
• NCBI Official Synonym Symbols: APS; PSA; hK3; KLK2A1
• NCBI Protein Information: prostate-specific antigen
• UniProt Protein Name: Prostate-specific antigen
• Protein Family: PsaJ-like protein asl3190
• UniProt Gene Name: KLK3
• UniProt Synonym Gene Names: APS; PSA; Seminin

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: EC 3.4.21.77; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular region; extracellular space; nucleus; protein complex

Molecular Function: endopeptidase activity; hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; protein binding; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: antibacterial peptide production; cellular protein metabolic process; negative regulation of angiogenesis; proteolysis