Chromatin Immunoprecipitation (ChIP)
(NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.)
Rabbit anti-Human NFkB p65 Polyclonal Antibody | anti-NFkBp65 antibody
NFkB p65 antibody (pAb)
ChIP-Seq: 10ul per ChIP
ChIP: 4ul per ChIP
WB: 1:2,500-1:5,000 dilution
Chromatin Immunoprecipitation (ChIP)
(NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.)
Chromatin Immunoprecipitation (ChIP)
(NFkB p65 antibody tested by ChIP-Seq. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 25 ug of chromatin from a human brain tumor and 10 ul of NFkB p65 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 9.5 million sequence tags were mapped to identify NFkB p65 binding sites on chromosome 19.)
Chromatin Immunoprecipitation (ChIP)
(NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(NFkB p65 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 2 million HT29 cells treated with TNF and 10 ul of p65 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Western Blot (WB)
(NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.)
Western Blot (WB)
(NFkB p65 (pAb) tested by Western blot. NFkB p65 detection by Western blot. The analysis was performed using 20 ug HeLa cytoplasmic extract and NFkB p65 pAb at a 1:2,500 dilution.)
Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.
NCBI and Uniprot Product Information
NCBI Description
NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.
Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; NF-kappaB binding; protein binding; transcription activator binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor activity; protein kinase binding; transcription factor binding
Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; cellular defense response; innate immune response; response to cobalamin
Research Articles on NFkBp65
Similar Products
Product Notes
The NFkBp65 rela (Catalog #AAA388460) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NFkB p65 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB p65 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB). Applications Validated: ChIP-Seq: 10ul per ChIP ChIP: 4ul per ChIP WB: 1:2,500-1:5,000 dilution. Researchers should empirically determine the suitability of the NFkBp65 rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
