Chromatin Immunoprecipitation (ChIP)
(p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 25 ug LNCaP cel extract and 5 ug of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify p300 binding sites on chromosome 7.)
Mouse anti-Human p300 Monoclonal Antibody | anti-p300 antibody
p300 antibody (mAb)(Clone NM11)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Western Blot
Purity
Protein A Chromatography
Synonyms
p300; Monoclonal Antibody; p300 antibody (mAb)(Clone NM11); EP300; KAT3B; RSTS2; anti-p300 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
NM11
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-p300 antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 5ug per ChIP
ChIP-Seq: 5ug each
ICC/IF: 0.5ug/ml dilution
ChIP: 5ug per ChIP
ChIP-Seq: 5ug each
ICC/IF: 0.5ug/ml dilution
Immunogen
The antibody was raised against full-length p300 purified from human 293 cells.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 25 ug LNCaP cel extract and 5 ug of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify p300 binding sites on chromosome 7.)
Chromatin Immunoprecipitation (ChIP)
(p300 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 25 ug LNCaP cel extract and 5 ug of p300 antibody. ChIP DNA was sequenced on the Illumina HiSeq and 6 million sequence tags were mapped to identify p300 binding sites on chromosome 7.)
Chromatin Immunoprecipitation (ChIP)
(p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 4.5 million LNCaP cells and 4 ug of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(p300 antibody (mAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with chromatin from 4.5 million LNCaP cells and 4 ug of p300 antibody. ChIP DNA was used in qPCR with the negative control primer pair or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Immunofluorescence (IF)
(p300 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 ug/ml dilution.)
Immunofluorescence (IF)
(p300 antibody (mAb) tested by immunofluorescence. Formaldehyde fixed HeLa cells stained with p300 antibody at a 0.5 ug/ml dilution.)
Related Product Information for anti-p300 antibody
Short Description: Our p300 antibody (mAb)(Clone NM11) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot; it has been shown to react with Human samples.
Background: p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
Background: p300 (E1A binding protein p300) functions as a histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Also functions as an acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator in the presence of CREBBP. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein.
Product Categories/Family for anti-p300 antibody
NCBI and Uniprot Product Information
Molecular Weight
300kDa
Similar Products
Product Notes
The p300 (Catalog #AAA388880) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The p300 antibody (mAb)(Clone NM11) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's p300 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Applications Validated: ChIP: 5ug per ChIP ChIP-Seq: 5ug each ICC/IF: 0.5ug/ml dilution. Researchers should empirically determine the suitability of the p300 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "p300, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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