Chromatin Immunoprecipitation (ChIP)
(GATA-1 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of K562 cells and 10 ug of GATA-1 antibody. ChIP DNA was used in qPCR with the gene-specific primer for PROX (a gene involved in heme biosynthesis that is regulated by GATA-1) or negative control primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Rabbit anti-Human GATA-1 Polyclonal Antibody | anti-GATA-1 antibody
GATA-1 antibody (pAb)
ChIP-Seq: 4ug per ChIP
ChIP: 10ug per ChIP
Chromatin Immunoprecipitation (ChIP)
(GATA-1 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of K562 cells and 10 ug of GATA-1 antibody. ChIP DNA was used in qPCR with the gene-specific primer for PROX (a gene involved in heme biosynthesis that is regulated by GATA-1) or negative control primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(GATA-1 (pAb) tested by ChIP. ChIP was performed using the ChIP-IT High Sensitivity Kit with 15 ug of K562 cells and 10 ug of GATA-1 antibody. ChIP DNA was used in qPCR with the gene-specific primer for PROX (a gene involved in heme biosynthesis that is regulated by GATA-1) or negative control primer as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(GATA-1 antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cells and 4 ug of GATA-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify GATA-1 binding sites on chromosome 1.)
Chromatin Immunoprecipitation (ChIP)
(GATA-1 antibody (pAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of K562 cells and 4 ug of GATA-1 antibody. ChIP DNA was sequenced on the Illumina NextSeq and 12 million sequence tags were mapped to identify GATA-1 binding sites on chromosome 1.)
Background: GATA-1 (Globin transcription factor) is a Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
NCBI and Uniprot Product Information
Uniprot Description
GATA1: Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells. May form homodimers or heterodimers with other isoforms. Interacts (via the N-terminal zinc finger) with ZFPM1. Interacts with GFI1B. Interacts with PIAS4; the interaction enhances sumoylation and represses the transactivational activity in a sumoylation-independent manner. Interacts with LMCD1. Interacts with CREBBP; the interaction stimulates acetylation and transcriptional activity in vivo. Interacts with EP300. Erythrocytes. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; chromatin DNA binding; p53 binding; sequence-specific DNA binding; DNA bending activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; erythrocyte development; embryonic hemopoiesis; megakaryocyte differentiation; in utero embryonic development; positive regulation of erythrocyte differentiation; eosinophil differentiation; male gonad development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; negative regulation of bone mineralization; positive regulation of peptidyl-tyrosine phosphorylation; cell-cell signaling; positive regulation of osteoblast proliferation; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; platelet formation; blood coagulation; basophil differentiation; negative regulation of apoptosis
Disease: Thrombocytopenia With Beta-thalassemia, X-linked; Anemia, X-linked, With Or Without Neutropenia And/or Platelet Abnormalities; Thrombocytopenia, X-linked, With Or Without Dyserythropoietic Anemia; Down Syndrome
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Product Notes
The GATA-1 gata1 (Catalog #AAA388940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GATA-1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GATA-1 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq). Applications Validated: ChIP-Seq: 4ug per ChIP ChIP: 10ug per ChIP. Researchers should empirically determine the suitability of the GATA-1 gata1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GATA-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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