Chromatin Immunoprecipitation (ChIP)
(MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.)
Mouse anti-Human MITF Monoclonal Antibody | anti-MITF antibody
MITF antibody (mAb)(Clone C5.D5)
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Gel Super Shift Assay, Western Blot
Purity
Protein G Chromatography
Synonyms
MITF; Monoclonal Antibody; MITF antibody (mAb)(Clone C5.D5); MI; WS2; CMM8; WS2A; bHLHe32; anti-MITF antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
C5.D5
Purity/Purification
Protein G Chromatography
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-MITF antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 2.5-10ug per ChIP
ChIP-Seq: 4ug each This
antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
ChIP: 2.5-10ug per ChIP
ChIP-Seq: 4ug each This
antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody
Immunogen
This MITF antibody was raised against an N-terminal fragment of human MITF.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.)
Chromatin Immunoprecipitation (ChIP)
(MITF antibody (mAb) tested by ChIP-Seq. (ChIP) was performed using the ChIP-IT High Sensitivity Kit and chromatin from a human melanoma cell line (2.5 million cells). ChIP DNA was sequenced on the Illumina GA II and 19 million sequence tags were mapped to identify MITF binding sites across the genome. The image shows hundreds of MITF binding sites across a 60 million bp region on chromosome 9.)
Chromatin Immunoprecipitation (ChIP)
(MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human lung melanoma cell line MALME-3M and 4 ug of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Chromatin Immunoprecipitation (ChIP)
(MITF antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from human lung melanoma cell line MALME-3M and 4 ug of MITF antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.)
Related Product Information for anti-MITF antibody
Short Description: Our MITF antibody (mAb)(Clone C5.D5) was raised in a Mouse host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Supershift and Western blot; it has been shown to react with Human samples.
Background: MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.
Background: MITF protein (Microphthalmia-associated Transcription Factor) is a basic helix-loop-helix type transcription factor involved in the differentiation and development of retinal pigment epithelium and melanocytes. It is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes tyrosinase and tyrosinase related protein 1. For activity MITF needs to heterodimerize with other bHLH proteins such as TFE3, TFEB or TFE. Defects in MITF are the cause of Waardenburg syndrome type 2A and Tietz syndrome.
Product Categories/Family for anti-MITF antibody
NCBI and Uniprot Product Information
Molecular Weight
52-56kDa
Protein Family
Similar Products
Product Notes
The MITF (Catalog #AAA388578) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MITF antibody (mAb)(Clone C5.D5) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MITF can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Electrophoretic Mobility Shift Assay (EMSA), Western Blot (WB). Applications Validated: ChIP: 2.5-10ug per ChIP ChIP-Seq: 4ug each This antibody is also available as an AbFlex engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody. Researchers should empirically determine the suitability of the MITF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MITF, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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