Rabbit anti-Human MCM8 Polyclonal Antibody | anti-MCM8 antibody

MCM8, ID (MCM8, C20orf154, DNA replication licensing factor MCM8, Minichromosome maintenance 8) (Biotin)

Cat. Num. AAA6319729

• Gene Names: MCM8; POF10; C20orf154; dJ967N21.5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCM8; Polyclonal Antibody; ID (MCM8; C20orf154; DNA replication licensing factor MCM8; Minichromosome maintenance 8) (Biotin); Minichromosome maintenance 8; anti-MCM8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 840

• Applicable Applications for anti-MCM8 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 321-351 amino acids from the Central region of human MCM8.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCM8 Antibody (Center) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the MCM8 antibody detected the MCM8 protein (arrow).)

Related Product Information for anti-MCM8 antibody

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the MCM proteins. This protein has been shown to co-immunoprecipitate with MCM4, 6 and 7, which suggests that it may interact with other MCM proteins and play a role in DNA replication. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Product Categories/Family for anti-MCM8 antibody

Antibodies; DNA Replication; Repair

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Pelak, K., et al. J. Infect. Dis. 201(8):1141-1149(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). He, C., et al. Nat. Genet. 41(6):724-728(2009). Stolk, L., et al. Nat. Genet. 41(6):645-647(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 19923727
• NCBI GeneID: 84515
• NCBI Accession #: NP_115874.3; NP_877954.1
• NCBI GenBank Nucleotide #: NP_115874.3
• UniProt Accession #: Q9UJA3
• NCBI Official Full Name: DNA helicase MCM8 isoform 1
• NCBI Official Synonym Full Names: minichromosome maintenance 8 homologous recombination repair factor
• NCBI Official Symbol: MCM8
• NCBI Official Synonym Symbols: POF10; C20orf154; dJ967N21.5
• NCBI Protein Information: DNA helicase MCM8
• UniProt Protein Name: DNA helicase MCM8
• Protein Family: DNA helicase
• UniProt Gene Name: MCM8
• UniProt Synonym Gene Names: C20orf154
• UniProt Entry Name: MCM8_HUMAN

NCBI Description

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the mini-chromosome maintenance proteins is a key component of the pre-replication complex and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein contains the central domain that is conserved among the mini-chromosome maintenance proteins. The encoded protein may interact with other mini-chromosome maintenance proteins and play a role in DNA replication. This gene may be associated with length of reproductive lifespan and menopause. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2013]

Uniprot Description

MCM8: Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle. Belongs to the MCM family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; EC 3.6.4.12

Chromosomal Location of Human Ortholog: 20p12.3

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; DNA binding; helicase activity; ATP binding

Biological Process: male gamete generation; female gamete generation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; response to DNA damage stimulus; double-strand break repair via homologous recombination; G1/S transition of mitotic cell cycle

Disease: Premature Ovarian Failure 10