Rabbit anti-Human NFkB p65 phospho Ser529 Polyclonal Antibody | anti-NFkB antibody

NFkB p65 phospho Ser529 antibody (pAb)

Cat. Num. AAA388543

• Gene Names: RELA; p65; CMCU; NFKB3
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: NFkB p65 phospho Ser529; Polyclonal Antibody; NFkB p65 phospho Ser529 antibody (pAb); RELA; p65; NFKB3; anti-NFkB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purified
• Form/Format: Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

• Applicable Applications for anti-NFkB antibody: Western Blot (WB)
• Application Notes: Applications Validated:; WB: 1:500-1:1,000 dilution
• Conjugation: Phosphorylated
• Immunogen: This NFkB p65 phospho Ser529 antibody was raised against a peptide containing phospho-serine 529 of human NFkB p65.
• Abs Residue: Ser
• Preparation and Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(NFkB p65 phospho Ser529 pAb tested by Western blot. HeLa cell extract (20 ug per lane) probed with either NFkB p65 phospho Ser529 pAb (1:1,000 dilution. Lane 1: extract derived from untreated cells. Lane 2: extract derived from cells treated with TNF-a and Calyculin.)

Dot Blot (DB)

(NFkB p65 phospho Ser529 pAb tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of of NFkB p65 phospho Ser529 pAb for NFkB p65 phosphorylated at serine 529. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with NFkB p65 phospho Ser529 pAb at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: phospho Ser276 NFkB p65 peptide. Lane 2: unmodified peptide surrounding Ser276 NFkB p65 peptide. Lane 3: phospho Ser529 NFkB p65 peptide. Lane 4: unmodified peptide surrounding Ser529 NFkB p65. Lane 5: phospho Ser536 NFkB p65 peptide. Lane 6: unmodified peptide surrounding Ser536 NFkB p65. Lane 7: phospho Ser337 NFkB p50 peptide. Lane 8: unmodified peptide surrounding Ser337 NFkB p50.)

Related Product Information for anti-NFkB antibody

Short Description: Our NFkB p65 phospho Ser529 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.

Background: NFkB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFkB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFkB p65, NFkB3) and RelB (I-Rel). Rel proteins form dimers with the NFkB p50 (proteolytically processed from p105/NFkB1) and p52 (proteolytically processed from p100/NFkB2) members of the NFkB family that bind DNA and activate transcription. The NFkB p65/p50 heterodimer is the most abundant form of NFkB. NFkB signaling is negatively regulated by the sequestration of the NFkB complex in the cytoplasm by its association with the IkB family of inhibitory proteins. The IkB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFkB. The IKK complex is composed of two catalytic subunits (IKKa and IKKb) and a regulatory subunit (IKKg). Upon its activation, the IKK complex phosphorylates IkB proteins (IkBa, IkBa phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFkB complex to translocate to the nucleus where it regulates transcription by binding to DNA.

Product Categories/Family for anti-NFkB antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 223468676
• NCBI GeneID: 5970
• NCBI Accession #: NP_068810; AB_2793307
• NCBI GenBank Nucleotide #: NM_021975
• UniProt Accession #: Q04206
• Molecular Weight: 60kDa
• NCBI Official Full Name: transcription factor p65 isoform 1
• NCBI Official Synonym Full Names: RELA proto-oncogene, NF-kB subunit
• NCBI Official Symbol: RELA
• NCBI Official Synonym Symbols: p65; CMCU; NFKB3
• NCBI Protein Information: transcription factor p65
• UniProt Protein Name: Transcription factor p65
• UniProt Gene Name: RELA
• UniProt Synonym Gene Names: NFKB3
• UniProt Entry Name: TF65_HUMAN

NCBI Description

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFkB-p65: a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified.

Protein type: Transcription factor; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; NF-kappaB binding; protein binding; transcription activator binding; DNA binding; protein heterodimerization activity; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor activity; protein kinase binding; transcription factor binding

Biological Process: viral reproduction; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; hair follicle development; toll-like receptor 4 signaling pathway; defense response to virus; response to drug; membrane protein intracellular domain proteolysis; positive regulation of I-kappaB kinase/NF-kappaB cascade; acetaldehyde metabolic process; response to amino acid stimulus; toll-like receptor 2 signaling pathway; organ morphogenesis; positive regulation of chondrocyte differentiation; response to muramyl dipeptide; response to mechanical stimulus; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; response to progesterone stimulus; negative regulation of protein catabolic process; negative regulation of apoptosis; transcription from RNA polymerase II promoter; response to cAMP; response to morphine; negative regulation of transcription from RNA polymerase II promoter; toll-like receptor 10 signaling pathway; response to insulin stimulus; response to organic substance; positive regulation of cell proliferation; positive regulation of interferon type I production; inflammatory response; aging; positive regulation of Schwann cell differentiation; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-12 biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; cellular defense response; innate immune response; response to cobalamin